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Nanotrap® Protocols - Virus Applications

Protocol I057: RNA Extraction from Clinical Swab Samples

Direct extraction of viral RNA from clinical swab samples using Nanotrap® Magnetic Virus Particles. 

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Summary: This document describes a protocol using Nanotrap® Magnetic Virus Particle to capture and concentration whole viruses from clinical swab samples followed by a direct RNA extraction. This method is compatible with clinical swab samplesstored in viral transport medium (VTM), universal transport media (UTM), or saline, as long as the medium used to collect the swabs and the storage conditions do not lyse viruses. This method has been confirmed with samples that contain infectious virus and with samples that contain virus that has been heat-inactivated at 65°C for 30 minutes.