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Nanotrap® Protocols - Virus Applications

Protocol I057: RNA Extraction from Clinical Swab Samples

Direct extraction of viral RNA from clinical swab samples using Nanotrap® Magnetic Virus Particles. 

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Summary: This document describes a protocol using Nanotrap® Magnetic Virus Particle to capture and concentration whole viruses from clinical swab samples followed by a direct RNA extraction. This method is compatible with clinical swab samplesstored in viral transport medium (VTM), universal transport media (UTM), or saline, as long as the medium used to collect the swabs and the storage conditions do not lyse viruses. This method has been confirmed with samples that contain infectious virus and with samples that contain virus that has been heat-inactivated at 65°C for 30 minutes.

SARS-CoV-2 Wastewater RNA Concentration and Extraction

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Summary: Medium throughput SARS-CoV-2 wastewater testing method developed by University of Connecticut

Protocol available on protocols.io 

Automated high throughput viral concentration from wastewater using the KingFisher Flex platform

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Summary: Large-scale wastewater surveillance has the ability to greatly augment the tracking of infection dynamics especially in communities where the prevalence rates far exceed the testing capacity. However, current methods for viral detection in wastewater are severely lacking in terms of scaling up for high throughput. In the present study, we employed an automated magnetic-bead based concentration approach for viral detection in sewage that can effectively be scaled up for processing 24 samples in a single 40-minute run. The method compared favorably to conventionally used methods for viral wastewater concentrations with a limit of detection of 8.809 viral gene copies/ml from input sample volumes as low as 10ml and can enable the processing of over 100 wastewater samples in a day.

Protocol available on protocols.io