Wyoming Department of Health uses Nanotrap Technology to improve the SARS-CoV-2 wastewater testing workflow
Microbes of Interest
influenza A and influenza B
Nanotrap® Microbiome A Particles
Nanotrap® Microbiome B Particles
Nanotrap® Enhancement Reagent 3
About the Lab
The wastewater-based epidemiology (WBE) testing team at the Wyoming Department of Health started running samples in August 2023 and are ramping up to run approximately 40 samples per week, coming from 4 sites.
The University of Wyoming has identical WBE protocols in place that will allow for additional surveillance and trending analysis to take place. The Wyoming Department of Health Wastewater Section is forming a statewide WBE committee, which is currently composed of 16 wastewater officials from the state and local levels to ensure they address the most important needs of the communities.
The Team (left to right): Cyrus Rautman, Scientist, Dallin North, Scientist, and Jim Mildenberger, Molecular Supervisor
Reduce time to concentrate samples: It took over 12 hours to process samples.
Remove the bottlenecks related to using the PEGs workflow: Centrifugation, the large volume process, (50 mL), and challenging pellet isolation
Simplify the workflow: To make it faster and easier to train new scientists at the lab and new university students
Future-proof the workflow: To be prepared to come online for new tests
Ability to multiplex every sample: To make it easier for multiple microbe detection from a single wastewater sample
1. Time savings: Able to process samples in 1 day, then look at the data and upload to NWSS the next day.
2. Confidence in the results: Inconsistencies are eliminated, the workflow is more fool-proof, and it is easier to train new technicians.
3. Multiplexing capable: Able to multiplex from a single sample.
4. Centrifugation is eliminated: Now able to test samples from more sites (previously limited to only 6-8 sites per week due to centrifugation limitations).
5. Saving on Costs: Pre-filtering and filtering samples is no longer necessary, resulting in both time and cost savings.
6. Lower sample volume requirements: 50 mL previous sample volume -> 10 mL current sample volume.
Capture & Concentration
A and B Particles
Nanotrap® Microbiome Enhancement Reagent 3
QIAGEN AllPrep PowerViral
DNA/RNA Kit on the QIACube
GT Molecular assays on the QIAcuity