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Wyoming Department of Health uses Nanotrap Technology to improve the SARS-CoV-2 wastewater testing workflow  

Microbes of Interest

SARS-CoV-2

influenza A and influenza B

RSV

norovirus

Candida Auris

Products

Nanotrap® Microbiome A Particles

Nanotrap® Microbiome B Particles

Nanotrap® Enhancement Reagent 3

About the Lab

The wastewater-based epidemiology (WBE) testing team at the Wyoming Department of Health started running samples in August 2023 and are ramping up to run approximately 40 samples per week, coming from 4 sites. 

The University of Wyoming has identical WBE protocols in place that will allow for additional surveillance and trending analysis to take place. The Wyoming Department of Health Wastewater Section is forming a statewide WBE committee, which is currently composed of 16 wastewater officials from the state and local levels to ensure they address the most important needs of the communities.

The Need

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The Team (left to right): Cyrus Rautman, Scientist, Dallin North, Scientist, and Jim Mildenberger, Molecular Supervisor 
 

  • Reduce time to concentrate samples: It took over 12 hours to process samples.

  • Remove the bottlenecks related to using the PEGs workflow: Centrifugation, the large volume process, (50 mL), and challenging pellet isolation

  • Simplify the workflowTo make it faster and easier to train new scientists at the lab and new university students

  • Future-proof the workflow: To be prepared to come online for new tests

  • Ability to multiplex every sample: To make it easier for multiple microbe detection from a single wastewater sample

The Results

 1. Time savings: Able to process samples in 1 day, then look at the data and upload to NWSS the next day.
2. Confidence in the results: Inconsistencies are eliminated, the workflow is more fool-proof, and it is easier to train new technicians.
3. Multiplexing capable: Able to multiplex from a single sample.
4. Centrifugation is eliminated: Now able to test samples from more sites (previously limited to only 6-8 sites per week due to centrifugation limitations).
5. Saving on Costs: Pre-filtering and filtering samples is no longer necessary, resulting in both time and cost savings.
6. Lower sample volume requirements: 50 mL previous sample volume -> 10 mL current sample volume.

The Solution

Capture & Concentration

Products

  • Nanotrap® Microbiome
    A and B Particles

  • Nanotrap® Microbiome Enhancement Reagent 3

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Extraction

Products

  • QIAGEN AllPrep PowerViral
    DNA/RNA Kit on the QIACube

Analysis

Products

  • GT Molecular assays on  the QIAcuity

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