In this study, we demonstrate that the Nanotrap® Protein Enrichment Affinity Kit (PEAK) is compatible with the PreOmics® iST kit for proteomic analysis of human plasma. TECH NOTE SKU # 34XXX Lit. # PL-TN31430

The Nanotrap® Protein Enrichment Affinity Kit (PEAK) uses magnetic hydrogel particle technology to capture and concentrate lowabundance proteins and peptides, leading to improved protein detection via LC-MS/MS. With three unique Nanotrap® Protein Particle chemistries available, researchers can easily adapt their approach based on specific protein targets or use case. Here we demonstrate that a simple, 45-minute enrichment process using the Nanotrap® PEAK is compatible with commercial digestion kits from four different vendors: Promega, Pierce, PreOmics, and Thermo Fisher Scientific. We also demonstrate that the Nanotrap PEAK significantly decreases the albumin levels in plasma samples. POSTER SKU # 34XXX Lit. # PL-PO31443

Detecting low-abundance biomarkers in complex biological samples is a persistent challenge in proteomics and clinical diagnostics. Conventional sample processing methods do not provide sufficient enrichment of biomarkers present in clinical sample matrices, which are often present at low concentrations. The Nanotrap® Protein Enrichment Affinity Kit (PEAK) enhances protein concentration and recovery. In this poster, we implemented the manual and automated Nanotrap® PEAK workflows, demonstrating a high-throughput method for protein biomarker enrichment that improves protein detection sensitivity from human K2EDTA plasma. POSTER SKU # 34XXX Lit. # PL-PO31444

Mass spectrometry-based proteomic analysis of plasma is a vital tool for biomarker discovery, yet it is hindered by high-abundance proteins, which can obscure the detection of low-abundance biomarkers. Nanotrap® Protein Enrichment Affinity Kit (PEAK)—simple, versatile, and easy-to-use kits that improve the detection of low-abundance proteins from multiple sample types. These kits utilize the Nanotrap® magnetic hydrogel particle technology to enrich low-abundance proteins from complex sample matrices. In this poster, three different Nanotrap® Protein Particle types and multiple particle combinations for plasma processing were evaluated. Each of the workflows offers unique benefits, allowing researchers to tailor their approach. Additionally, we investigated whether a simple 30-minute enrichment step using Nanotrap® PEAK would enhance protein identification in plasma, cerebrospinal fluid (CSF), and urine samples. POSTER SKU # 34XXX Lit. # PL-PO31442

In this study, we demonstrate that the Nanotrap® Protein Enrichment Affinity Kit (PEAK) is compatible with the Pierce™ In-Solution Tryptic Digestion and Guanidination Kit for proteomic analysis of human plasma. TECH NOTE SKU # 34XXX Lit. # PL-TN31431

Mpox (formerly known as monkeypox) has historically been present in western and central Africa. However, global outbreaks in May 2022 and August 2024 prompted the World Health Organization (WHO) to declare public health emergencies of international concern. The 2022 outbreak was linked to Clade II Mpox. The ongoing 2024 outbreak is driven by a newly identified strain of Clade I Mpox, known as Clade Ib. Clade I is believed to have a higher fatality rate than clade II. Clade Ib was first detected in South Kivu, in the Democratic Republic of the Congo, and now multiple strains of Clade Ib are circulating.(1,2)  Clade Ib Mpox is difficult to diagnose based on clinical symptoms alone, as its visual presentation is not distinct enough for reliable identification.(3) This variant includes a deletion in a genomic region previously targeted by diagnostic tests,(4) which may contribute to underreporting of cases. However, sequencing data from Clade Ib strains have enabled the development of a new PCR-based assay for detecting Mpox in wastewater. This assay targets a region of the genome that is not affected by the deletion, offering a promising tool for monitoring community infection levels and tracking the spread of the disease. POSTER SKU 44XXX

Surveillance of zoonotic influenza A viruses, including highly pathogenic H5N1, has gained urgency in light of potential risks to food safety and public health.(1) Milk—particularly raw or low-pasteurized forms—has been identified as a possible transmission route for influenza A viruses originating in livestock.(2,3) However, current molecular workflows often rely on small input volumes (e.g., 25 µL) and do not account for matrix complexity, limiting sensitivity and genome coverage for sequencing-based detection.(2) To address these limitations, we developed an enhanced method for influenza A virus detection and sequencing from milk using Nanotrap® Microbiome A Particles. This workflow was tested using H1N1 influenza A virus and compared against a direct extraction method. Paired with qPCR and whole-genome sequencing, this approach offers a scalable solution to improve sensitivity and genome recovery from complex dairy matrices. In this poster, we examine whether Nanotrap Microbiome A Particles improve the detection sensitivity of the Influenza A virus from milk relative to current standard extraction methods. POSTER SKU 44XXX

The adoption of wastewater-based epidemiology (WBE) for monitoring SARS-CoV-2 has rapidly expanded worldwide, driven by strong evidence linking WBE and clinical case trends. The success of WBE for monitoring of SARS-CoV-2 prevalence in specific regions has led to a renewed interest in monitoring additional microorganisms, including yeast, such as Candida auris ( C. auris ). Transmission of Candida  species is oftentimes linked to physical contact with an infected host or through direct contact with a contaminated surface, particularly in hospitals and healthcare facilities.(1)  C. auris  has also been observed to contain genes which confer antifungal resistance,(2) leading to the further need for broad surveillance of infectivity rates due to the limited treatment options available. Detecting these microbes also presents challenges; they contain strong cell structures and are difficult to lyse using common nucleic acid extraction workflows, and their concentration in wastewater is often very low due to infection control measures in healthcare settings. Culture-based methods have proven effective for detecting and characterizing C. auris in wastewater, improving the ability to study its infectivity at the community level. POSTER SKU 44XXX

Wastewater-based epidemiology (WBE) is a powerful tool for population-level monitoring of respiratory viruses, enabling early detection of circulating pathogen strains and variant emergence.(1–3) However, sequencing viruses like influenza A and respiratory syncytial virus (RSV) from wastewater remains technically challenging due to low viral titers, fragmented RNA, and high background from environmental and host nucleic acids.(1,2) Traditional sequencing workflows often yield incomplete genomes, limiting their utility for surveillance and variant tracking.(3) To address these challenges, we applied an enrichment strategy using Nanotrap® Microbiome A Particles with a magnetic bead-based extraction, followed by virus-specific targeted sequencing workflows.(4) For influenza A, we used the NEBNext® iiMS DNA Library Prep Kit with Oxford Nanopore sequencing. For RSV, targeted amplicon generation was performed using NEBNext RSV Primers and Illumina library prep. This combined approach supports high-throughput, same-day sequencing of respiratory viruses from wastewater. POSTER SKU 44XXX

Product: Nanotrap® Protein Enrichment Affinity Kit In this study, we demonstrate that the Nanotrap® Protein Enrichment Affinity Kit (PEAK) is compatible with the Thermo Scientific™ SMART Digest™ Trypsin Kit for proteomic analysis of human plasma. APPLICATION NOTE SKU # 34XXX Lit. # PL-TN31432

In proteomics, achieving high reproducibility and deep proteome coverage is essential for confident biomarker discovery, especially when working with complex biological samples like human plasma. One of the most critical metrics for assessing reproducibility is the coefficient of variation (CV), which quantifies the consistency of protein quantification across replicate runs. Lower CVs translate to greater confidence in detected differences and reduce the number of replicates needed to achieve statistical significance, saving both time and resources.(1) In this study, we demonstrate that the Nanotrap® Protein Enrichment Affinity Kit (PEAK)—applied through both manual and semi-automated protocols—enables substantial improvements in both proteome coverage and technical reproducibility.(2,3) Compared to neat plasma processing, the Nanotrap PEAK manual and KingFisher™ Apex System protocols reduced median protein CVs from 13% to 8.4% and 2.3%, respectively. This reduction represents up to a 32-fold decrease in the number of replicates required to achieve equivalent statistical power. These results establish Nanotrap PEAK workflows as highly efficient and reproducible solutions for plasma proteomics, supporting both deep discovery and robust quantitation. APPLICATION NOTE SKU # 34XXX Lit. # PL-AN31416

In this study, we demonstrate that protein enrichment from human plasma using the Nanotrap® Protein Enrichment Affinity Kit (PEAK) can be performed using semi-automated protocols on the KingFisher™ Flex and KingFisher™ Apex Systems. TECH NOTE SKU # 34XXX Lit. # PL-TN31429