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Customer Advance
UC Davis Proteomics Core Facility

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The UC Davis Proteomics Core lab offers state-of-the-art LC-MS/MS analysis, including protein identification, proteomics profiling, targeted proteomics, and post-translational modification analysis, for both on- and off-campus clients.


Samples run include plasma/serum, tissues, cell culture/pellets, plant material, powder and more.

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The Need

  • Decreased Albumin and Immunoglobulins (IgGs) to enable better detection of the interesting proteins

  • Simple, easy-to-understand workflow
    with fewer sample prep and clean-up
    steps

  • More reproducible results

  • No sample loss (Concern of sample loss
    due to adding perchloric acid step in
    other protocols)

  • Ability to scale up in terms of cost
    and time

“What really struck me when I first looked at the protocol was how straightforward it seemed. Just the preparation (and) the difference in the number of preparation steps made it feel much more approachable than some of the other protocols that I’ve done.”

– Lauren Dixon, Staff Researcher, UC Davis Proteomics Core Facility
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Results

  • Reduced interference from Albumin and IgGs

  • Straightforward workflow with fewer steps from
    sample through enrichment

  • Wash step makes it more compatible with digestion and analysis steps – no proprietary wash solutions required

  • Affordable cost per sample enables scale-up to larger studies

  • Confirmed reproducibility of results

  • Less sample loss due to elimination of the clean-up step, as compared to Preomics digestions and Perchloric Acid where failure to clean up Perchloric Acid for depletion can be problematic

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The Solution

Enrichment

Typical tryptic digest 

conditions to cut down on extra steps and clean-ups

LCMS Analysis

Capture and

Concentration

Nanotrap® Protein
Enrichment Affinity Kit

  • Proteins Denatured in 5% SDS 50mM Ammonium Bicarbonate - compatible with S-Trap digestion (Protifi) or MagResyn PAC digestion

  • Reduction by dithiothreitol (DTT) and Alkylation with iodoacetamide (IAA)

  • Digestion: Trypsin

  • De-salting is not required since clean-up is “in-built” with S-Trap, and LCMS compatible solutions are used for digestions and elution

Evosep One and
timsToF HT

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