In this study, we evaluated three commercial cfDNA isolation kits to gauge their effectiveness in enriching and isolating cfDNA for colorectal cancer (CRC) detection via epigenetic screening. Methylation-specific real-time polymerase chain reaction (MSP RT-PCR) and high-resolution melting (HRM) results were used to compare the Nanotrap® Extraction Advanced Technology Liquid Biopsy Kit (NEAT Kit) to two other commercial kits for cfDNA extraction. Mock cfDNA samples were create

Vector-borne diseases, including yellow fever, Zika, chikungunya, West Nile, and dengue, are posing increasing health risks globally.(1) There is a need for a simple and effective workflow that can be used with wastewater samples to capture and concentrate multiple microbes from a single sample. This experiment demonstrates that the Nanotrap® Microbiome Workflow effectively captures and concentrates low-abundance, vector-borne disease microbes from a single wastewater sample.

This study addresses these challenges by using Nanotrap® Microbiome B Particles to enhance Mycobacterium capture and concentration from sputum, offering a novel approach for TB detection.