INTRODUCTION Plasma proteomics is inherently challenging due to its extreme dynamic range, with a small number of highly abundant proteins dominating the sample and limiting detection of lower-abundance, biologically relevant proteins. Enrichment strategies are therefore needed to increase proteome depth without introducing excessive variability, cost, or workflow complexity. The Nanotrap® Protein Enrichment Affinity Kit (PEAK) - Discovery uses magnetic hydrogel particles to

Mass gatherings increase the risk of infectious disease transmission, particularly when healthcare systems are overstretched and underfunded. Wastewater and environmental surveillance (WES) offer a real-time, non-invasive method for monitoring pathogen circulation at the population level. During Uganda’s 2025 Martyrs’ Day pilgrimage, which drew over three million people, the Ministry of Health used WES to identify and respond to health threats.

In this study, we demonstrate that the Nanotrap® Protein Enrichment Affinity Kit (PEAK) significantly reduces the levels of albumin in human plasma samples, as measured by LC-MS/MS. Figure Description: MaxLFQ intensity profiles for albumin across all Nanotrap® PEAK enrichment methods versus unenriched samples. Nanotrap PEAK-enriched samples consistently yielded lower albumin intensity across different LC gradient lengths. This demonstrates the robustness of the Nanotrap PEAK