Through the Nanotrap Microbiome A Particle workflow paired with downstream TAC analysis, we aim to demonstrate concentration across a wide range of infectious pathogen targets from wastewater samples gathered in various locations in Dhaka, Bangladesh.

Detection and isolation of intact bacteria from clinical specimens such as whole blood and urine are critical for clinical microbiology and translational research. However, complex matrix effects, low microbial burden, and the absence of sample preparation workflows that enable intact bacterial capture limit downstream analysis.

Evaluation of Nanotrap® Microbiome Particles, magnetic hydrogel nanoparticles previously validated for capturing viruses and bacteria in environmental water and clinical specimens, for the capture and concentration of arboviruses and Vibrio cholerae from environmental water.

This study presents an enhanced workflow using Nanotrap Microbiome A and Nanotrap Microbiome B Particles for viral enrichment with the NEBNext® Flu A Integrated Indexing Primer Module protocol, leveraging Oxford Nanopore sequencing for rapid whole-genome characterization. By integrating targeted cDNA synthesis and native barcoding, this approach enables same-day sequencing with improved genome coverage, offering a scalable and high-throughput solution for monitoring influenza

This study looks at fecal markers in surface water contaminated with human wastewater and utilizes a magnetic Nanotrap® Particle for capture and concentration of microbes in these samples for fast and reliable detection. This method is compared to a standard filtration method to determine relative sensitivity.