Utilizing TaqMan Array Card Assays for In-Depth Analysis of Wastewater Collected in Dhaka, Bangladesh

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Wastewater-based epidemiology (WBE) is a vital tool for tracking pathogenic outbreaks and has been utilized to detect a wide variety of microbes present within a community. In Dhaka, Bangladesh, WBE has been utilized to perform surveillance for poliovirus, other enteric pathogens, and known antimicrobial resistance markers. Extensive research has also been conducted on the persistence of human enteric viruses transmitted via the fecal-oral route in wastewater.

Nanotrap® Microbiome A Particles are functionalized hydrogel particles that bind and concentrate intact viruses, bacteria, fungi, and parasites from diverse sample matrices, and have been adopted in wastewater testing due to reduced workflow times and compatibility with automated, high-throughput processing. We evaluated microbe concentration using the Nanotrap Microbiome A Particle workflow paired with TaqMan Array Cards (TAC), which

are customizable RT-qPCR arrays on microfluidics cards, capable of interrogating 48+ pathogen targets per sample and enabling expanded multiplex potential.

Through the Nanotrap Microbiome A Particle workflow paired with downstream TAC analysis, we aim to demonstrate concentration across a wide range of infectious pathogen targets from wastewater samples gathered in various locations in Dhaka, Bangladesh.

Poster presented at the 2026 ASM Conference.

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