Evaluation of the Nanotrap PEAK Discovery Method, which uses magnetic hydrogel particles to enrich low abundance proteins from biofluids while depleting high-abundance interferents

Evaluation of Nanotrap® Protein Enrichment Affinity Kit (Nanotrap® PEAK) as a universal protein enrichment solution across multiple blood collection formats—including K2EDTA, K3EDTA, sodium citrate (NaCit), and hemolyzed samples—as well as non-human plasma from mouse and canine sources. The results highlight the flexibility of Nanotrap PEAK to support consistent protein enrichment across diverse matrices—helping enable more robust and scalable proteomics workflows.

Evaluation of 10 μL Nanotrap® PEAK Discovery Method against the standard 50 μL workflow on matched K2EDTA plasma samples.

Mass spectrometry–based proteomic analysis is a powerful tool for biomarker discovery, but the detection of low-abundance proteins in complex biological samples such as plasma, serum, cerebrospinal fluid (CSF), and urine remains challenging due to high dynamic range and matrix-specific workflow constraints. The presence of abundant proteins, such as albumin, can mask lower-abundance analytes, limiting proteome depth and sensitivity for clinically relevant targets, including c

INTRODUCTION Plasma proteomics is inherently challenging due to its extreme dynamic range, with a small number of highly abundant proteins dominating the sample and limiting detection of lower-abundance, biologically relevant proteins. Enrichment strategies are therefore needed to increase proteome depth without introducing excessive variability, cost, or workflow complexity. The Nanotrap® Protein Enrichment Affinity Kit (PEAK) - Discovery uses magnetic hydrogel particles to