Nanotrap Protein Enrichment Affinity Kits Outperform a Bead-Based Protein Enrichment Kit

Application Note -- Nanotrap Protein Enrichment Affinity Kits Outperform a Bead-Based Prot.

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In this study, the Nanotrap® Protein Enrichment Affinity Kit (PEAK) was compared to a magnetic, protein-binding

bead-based enrichment kit. Three different healthy human plasma samples were processed using several different Nanotrap® PEAK methods, including the Combined Particle Method, the 2-Particle Method, and the 3-Particle Method.1 After Nanotrap PEAK enrichment, each sample was digested, cleaned up, and analyzed using

LC-MS/MS. Each of the three samples was also processed using off-the-shelf kits for protein enrichment, digestion,

and clean-up and was analyzed using the same settings on the LC-MS/MS. Each of the three samples was also

processed without protein enrichment as a neat sample. Neat samples were digested, cleaned up, and analyzed

using LC-MS/MS.

All three of the Nanotrap PEAK methods resulted in more unique protein identifications than the competitor kit’s

method for all plasma samples.

APPLICATION NOTE SKU 34XXX

Lit # PL-AN31399