Reagents

Nanotrap® Enhancement Reagent 1

​

Nanotrap® Magnetic Virus Particles rapidly capture and concentrate viruses from raw sewage, requiring no filtration or centrifugation. Nanotrap® Enhancement Reagent 1 (ER1) improves the binding of viruses to Nanotrap® Magnetic Virus Particles in wastewater samples, further improving downstream detection of viral RNA. Automated and manual methods are available.

   PRODUCT DESCRIPTION

​

• Improve SARS-CoV-2 RNA detection in wastewater by at least 1-2 Ct values using Nanotrap® Enhancement Reagent 1 with Nanotrap® Magnetic Virus Particles

​

• No negative impact on recovery of control viruses (demonstrated with PMMoV)

​

• Easily amenable to automation when used with Nanotrap® Magnetic Virus Particles

​

• Convenient workflow: add to your wastewater sample at the same time as Nanotrap® Particles or add to your wastewater samples up to 18 hours before you add Nanotrap® Particles

​​

• Non-hazardous, non-perishable reagent, room temperature storage

​​

• Demonstrated compatibility with the following magnetic bead RNA extraction kits:

​​​

• MACHERY-NAGEL NucleoMag DNA/RNA Water Kit (Cat# 744220.1)

• Applied Biosystems™ MagMAX™ Viral/Pathogen Nucleic Acid Extraction Kit (Cat# A42352)

• Applied Biosystems™ MagMAX™ Viral/Pathogen II (MVP II) Nucleic Acid Extraction Kit (Cat# A48383)

• Applied Biosystems™ MagMAX™ Microbiome Ultra Nucleic Acid Kit (Cat# A42357)

​

​

Using Nanotrap Enhancement Reagent 1 with Nanotrap Particles improves detection of SARS-CoV-2 in raw wastewater samples when used with the NucleoMag DNA/RNA Water Kit. A KingFisher Apex System was used for virus capture and concentration, as well as for RNA extraction. Viral RNA was detected with the Promega N1 primer.

Using Nanotrap Enhancement Reagent 1 with Nanotrap Particles improves detection of PMMoV in raw wastewater samples when used with the NucleoMag DNA/RNA Water Kit. A KingFisher Apex System was used for virus capture and concentration, as well as for RNA extraction. Viral RNA was detected with the Promega N1 primer.

Incorporating the Nanotrap® technology into your wastewater testing is simple.