Reagents

Nanotrap® Enhancement Reagent 1

Nanotrap® Magnetic Virus Particles rapidly capture and concentrate viruses from raw sewage, requiring no filtration or centrifugation. Nanotrap® Enhancement Reagent 1 (ER1) improves the binding of viruses to Nanotrap® Magnetic Virus Particles in wastewater samples, further improving downstream detection of viral RNA. Automated and manual methods are available.

   PRODUCT DESCRIPTION

  • Improve SARS-CoV-2 RNA detection in wastewater by at least 1-2 Ct values using Nanotrap® Enhancement Reagent 1 with Nanotrap® Magnetic Virus Particles

  • No negative impact on recovery of control viruses (demonstrated with PMMoV)

  • Easily amenable to automation when used with Nanotrap® Magnetic Virus Particles

  • Convenient workflow: add to your wastewater sample at the same time as Nanotrap® Particles or add to your wastewater samples up to 18 hours before you add Nanotrap® Particles

  • Non-hazardous, non-perishable reagent, room temperature storage

  • Demonstrated compatibility with the following magnetic bead RNA extraction kits:

  • MACHERY-NAGEL NucleoMag DNA/RNA Water Kit (Cat# 744220.1)

  • Applied Biosystems™ MagMAX™ Viral/Pathogen Nucleic Acid Extraction Kit (Cat# A42352)

  • Applied Biosystems™ MagMAX™ Viral/Pathogen II (MVP II) Nucleic Acid Extraction Kit (Cat# A48383)

  • Applied Biosystems™ MagMAX™ Microbiome Ultra Nucleic Acid Kit (Cat# A42357)

Using Nanotrap Enhancement Reagent 1 with Nanotrap Particles improves detection of SARS-CoV-2 in raw wastewater samples when used with the NucleoMag DNA/RNA Water Kit. A KingFisher Apex System was used for virus capture and concentration, as well as for RNA extraction. Viral RNA was detected with the Promega N1 primer.

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Using Nanotrap Enhancement Reagent 1 with Nanotrap Particles improves detection of PMMoV in raw wastewater samples when used with the NucleoMag DNA/RNA Water Kit. A KingFisher Apex System was used for virus capture and concentration, as well as for RNA extraction. Viral RNA was detected with the Promega N1 primer.

ER1 PMMoV.png

Incorporating the Nanotrap® technology into your wastewater testing is simple. 

ER1 workflow.jpg

AVAILABLE PRODUCTS

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Nanotrap® Enhancement Reagent 1 (ER1)

Ready to ship in 10 mL vials. Use 0.1 mL for each 10 mL wastewater sample.
 
SKU# 10111