Reagents

Nanotrap® Enhancement Reagents

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Nanotrap® Magnetic Virus Particles rapidly capture and concentrate viruses from raw sewage, requiring no filtration or centrifugation. Nanotrap® Enhancement Reagent 1 (ER1) and Nanotrap® Enhancement Reagent 2 (ER2) improve the binding of viruses to Nanotrap® Magnetic Virus Particles in wastewater samples, further improving downstream detection of viral RNA. Automated and manual methods are available.

Incorporating the Nanotrap® technology into your wastewater testing is simple. 

   PRODUCT DESCRIPTION

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• Improve SARS-CoV-2 RNA detection in wastewater by at least 1-2 Ct values using Nanotrap® Enhancement Reagents with Nanotrap® Magnetic Virus Particles

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• No negative impact on recovery of control viruses 

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• Automation friendly when paired with Nanotrap® Magnetic Virus Particles

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• Convenient workflow: add to your wastewater sample at the same time as Nanotrap® Particles or add to your wastewater samples up to 18 hours before you add Nanotrap® Particles

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• Non-hazardous, non-perishable reagents, room temperature storage

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• Nanotrap Enhancement Reagent 1 is compatible with the following extraction kits:

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• MACHERY-NAGEL NucleoMag DNA/RNA Water Kit (Cat# 744220.1)

• Applied Biosystems™ MagMAX™ Viral/Pathogen Nucleic Acid Extraction Kit (Cat# A42352)

• Applied Biosystems™ MagMAX™ Viral/Pathogen II (MVP II) Nucleic Acid Extraction Kit (Cat# A48383)

• Applied Biosystems™ MagMAX™ Microbiome Ultra Nucleic Acid Kit (Cat# A42357)

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• Nanotrap Enhancement Reagent 2 is compatible with the following extraction kits:​​

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• QIAGEN MagAttract® Viral RNA Kit (960) (Cat# 955538)

• QIAGEN QIAamp® Viral RNA Mini Kit (250) (Cat# 52906)

• QIAGEN AllPrep® PowerViral® DNA/RNA Kit (50) (Cat# 28000-50)

• Zymo Research ZymoBIOMICS ™ MagBead DNA/RNA Kit (Cat# R2135)

• Promega Maxwell® HT Environmental TNA Kit, Custom (Cat# AX9190)

• IDEXX Water DNA/RNA Magnetic Bead Kit (Cat# 98-0014719-00)

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• For Research Use Only. Not for use in diagnostic procedures.

• All trademarks are the property of their respective owners.

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Using Nanotrap Enhancement Reagent 1 with Nanotrap Particles improves detection of SARS-CoV-2 in raw wastewater samples when used with the NucleoMag DNA/RNA Water Kit. A KingFisher Apex System was used for virus capture and concentration, as well as for RNA extraction. Viral RNA was detected with the Promega N1 primer.

Using Nanotrap Enhancement Reagent 1 with Nanotrap Particles improves detection of PMMoV in raw wastewater samples when used with the NucleoMag DNA/RNA Water Kit. A KingFisher Apex System was used for virus capture and concentration, as well as for RNA extraction. Viral RNA was detected with the Promega PMMoV primer.

Using Nanotrap Enhancement Reagent 2 with Nanotrap Particles improves detection of SARS-CoV-2 in raw wastewater samples when used with the QIAamp® Viral RNA Mini Kit. A KingFisher Apex System was used for virus capture and concentration, as well as for RNA extraction. Viral RNA was detected with the Promega N1 primer.

Using Nanotrap Enhancement Reagent 2 with Nanotrap Particles improves detection of PMMoV in raw wastewater samples when used with the QIAamp® Viral RNA Mini Kit. A KingFisher Apex System was used for virus capture and concentration, as well as for RNA extraction. Viral RNA was detected with the Promega PMMoV primer.