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A Sample-to-Results Workflow for RSV Sequencing from Wastewater

  • 22 hours ago
  • 1 min read


This application note outlines a scalable and automation-friendly workflow for respiratory syncytial virus (RSV) surveillance from wastewater, utilizing Ceres' Nanotrap® Microbiome A Particles for viral enrichment, New England Biolabs' Monarch® Mag Viral DNA/RNA Extraction Kit with DNase treatment to reduce gDNA contamination, and NEBNext® targeted sequencing for high genome coverage and accurate strain identification. ​ The workflow addresses challenges of low viral loads and wastewater variability, providing a cost-effective solution for public health surveillance and vaccine development. ​


A sample-to-results workflow for RSV sequencing from wastewater. A scalable, robust, and automation friendly workflow for respiratory syncytial virus surveillance, leveraging Nanotrap Microbiome A Particles, Monarch Mag Viral DNA/RNA extraction, and NEBNext RSV sequencing.
A sample-to-results workflow for RSV sequencing from wastewater. A scalable, robust, and automation friendly workflow for respiratory syncytial virus surveillance, leveraging Nanotrap Microbiome A Particles, Monarch Mag Viral DNA/RNA extraction, and NEBNext RSV sequencing.


TECH NOTE SKU # 34XXX



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