Capture and Detection of Mycobacterium from Sputum Samples using Nanotrap Microbiome B Particles

5 hours ago
1 min read

Tuberculosis (TB) remains a leading global health concern, causing approximately 1.4 million deaths annually.(1) Early and accurate detection of Mycobacterium tuberculosis in complex clinical samples, such as sputum, is critical for effective diagnosis and treatment. Traditional culture methods, while highly specific, are slow and labor-intensive. Molecular approaches such as qPCR and sequencing provide faster results but are often hindered by inefficient mycobacterial recovery and the presence of inhibitors in sputum.(2)

To address these limitations, we investigate the use of the Nanotrap® Microbiome B Particles. The particles are composed of a hydrogel matrix and functionalized with affinity bait molecules that capture and concentrate intact microbes from various sample matrices. We demonstrate that this approach can effectively capture and concentrate Mycobacterium directly from sputum, improving sensitivity and reducing workflow complexity.