Performance Comparison of Two MagMAX™ Extraction Kits Using the Nanotrap Microbiome Particle Workflow

Caryn Tyler
Jan 6
1 min read

Nanotrap® Microbiome Particles are widely used in wastewater surveillance to capture and concentrate microbial pathogens, enhancing the sensitivity and reliability of pathogen detection. This approach enables effective monitoring of microbes, providing valuable insights for public health initiatives. Following the concentration step, efficient nucleic acid extraction is critical to ensure the recovery of high-quality RNA and DNA for downstream molecular applications.

The MagMAX™ Wastewater Ultra Nucleic Acid Isolation Kit is an updated version of the MagMAX™ Microbiome

Ultra Nucleic Acid Isolation Kit.

The MagMAX Wastewater Ultra Nucleic Acid Isolation Kit protocol includes updates to reagent volumes, temperature conditions, and protocol steps, with key changes occurring in the binding and wash steps, completely removing Wash Step 3. The removal of Wash Step 3 results in time savings of approximately 5 minutes. The updated MagMAX protocol does not include reagents for bead beating. In this technical note, we show that the updated MagMAX Wastewater Ultra Nucleic Acid Isolation Kit protocol yields similar results to the MagMAX™ Microbiome Ultra Nucleic Acid Kit.