Improved methodology for detection of antibiotic resistance mechanisms from wastewater for population surveillance APPLICATION NOTE SKU 10XXX SKU 44XXX Download full application note here Introduction Recently, wastewater surveillance has been recognized as a complement to population testing that can identify a broad range of targets associated with AMR, and the methods must be sensitive given the larger wastewater comparison of the ability of commercially available real-time PCR-based assays to determine AMR burden in wastewater

treatment failures, coastal surface waters are subject to increased fecal contamination via domestic wastewater

the systematic collection of samples and associated data on infectious-disease-related pathogens from wastewater have started to implement environmental surveillance networks for the use of multi-pathogen data from wastewater

Poster: Wastewater-based epidemiology has been widely used to monitor COVID-19 trends in communities, Variants have been successfully identified and quantified in wastewater using next-generation sequencing Samples were collected weekly from the influent of several wastewater treatment facilities, public schools Omicron sub-lineage detection using the assays was aligned well with the NGS results from the same wastewater Wastewater samples collected between mid-February 2022 and mid-April 2022 showed a rapid displacement

Optimization of Nanotrap® Microbiome Particle methods for Concentrating SARS-CoV-2 from Small Volume Wastewater July 2023 Introduction: Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) RNA monitoring in wastewater Grab (quantitative) and passive samples (qualitative) are two distinct wastewater sampling methods. Results: Adding ER1 to wastewater prior to viral concentration significantly improved viral concentration Spiking known genome copies (GC) of inactivated SARS-CoV-2 into 10 mL wastewater indicated that the limit

Environment, June 2023 "Unveiling indicator, enteric, and respiratory viruses in aircraft lavatory wastewater Nanotrap® Microbiome A Particles workflows" Abstract: The effective detection of viruses in aircraft wastewater prevalence and concentrations of 15 endogenous viruses including ssDNA, dsDNA, ssRNA in 24 aircraft lavatory wastewater crAssphage), human polyomavirus (HPyV), rhinovirus A (RhV A), and rhinovirus B (RhV B) were detected in all wastewater

"Intensity of sample processing methods impacts wastewater SARS-CoV-2 whole genome amplicon sequencing outcomes" Science of The Total Environment, June 2023 ABSTRACT: Wastewater SARS-CoV-2 surveillance has Genomic surveillance of SARS-CoV-2 in wastewater, particularly the efforts for whole genome sequencing The intrinsic sample limitations are inherent to wastewater. samples, played important roles in wastewater SARS-CoV-2 amplicon sequencing.

medRxiv May 2023 Background The origin of divergent SARS-CoV-2 spike sequences found in wastewater, but These “cryptic” wastewater sequences have harbored many of the same mutations that later emerged in Omicron We first detected a cryptic lineage in municipal wastewater in Wisconsin in January 2022. Findings The persistence of the Wisconsin Lineage signal allowed us to trace it from a central wastewater Interpretation We propose that prolonged detection of the Wisconsin Lineage in wastewater represents

medRxiv, May 2023 "Human norovirus (HuNoV) GII RNA in wastewater solids at 145 United States wastewater Wastewater monitoring for HuNoV has been done previously and results indicate it is present in wastewater However, work has mostly been limited to monthly samples of liquid wastewater at one or a few wastewater Methods: We measured HuNoV GII RNA in matched wastewater solids and liquid influent in 7 samples from We also applied the HuNoV GII assay to measure viral RNA in over 6000 wastewater solids samples from

Poster: COVID-19 highlighted the use of wastewater testing as a complementary method to monitor emerging As a strategy to complement population testing, wastewater samples can be evaluated to identify increases identify a broad range of targets associated with AMR, and the methods must be sensitive given the larger wastewater head-to-head comparison of commercially available real-time PCR-based assays to assess AMR burden in wastewater

Quantifying Respiratory Viruses in Wastewater Using GT Molecular's Digital PCR Assays coupled with Ceres Acid Isolation Kit, and the KingFisher™ Apex Extraction Workflow™ APPLICATION NOTE SKU 10XXX SKU 44XXX Wastewater testing continues to gain traction as a viable means to monitor pathogens and provide timely Multiple countries have implemented wastewater-based monitoring of SARS-CoV-2, and the COVID-19 case rates highly correlate with SARS-CoV-2 RNA found in wastewater.

Quantifying Respiratory Viruses in Wastewater Using GT Molecular’s Digital PCR Assays coupled with Ceres and QIAGEN AllPrep PowerViral™ DNA/RNA Extraction Kit on the QIAcube™ APPLICATION NOTE SKU 10XXX SKU 44XXX Wastewater-based epidemiology (WBE) utility has already been successfully described to track infectious Multiple countries have implemented wastewater-based monitoring of SARS-CoV-2, and the COVID-19 case rates highly correlate with SARS-CoV-2 RNA found in wastewater.