Quantifying Respiratory Viruses in Wastewater Using GT Molecular’s Digital PCR Assays coupled with Ceres Nanosciences' Nanotrap® Workflow and QIAGEN AllPrep PowerViral™ DNA/RNA Extraction Kit on the QIAcube™
APPLICATION NOTE SKU 10XXX SKU 44XXX
Key Advantages
> High-throughput multiplex detection coupling GT Molecular’s Digital PCR Assays with the Nanotrap Particle Capture and Concentration Methods
> Ability to co-monitor and co-quantify multiple pathogens from a single sample
> Assays available for the QIAcuity® Platform
> Respiratory virus detection including: Influenza A, Influenza B and RSV in addition to SARS-CoV-2.
Introduction
Monitoring the spread of SARS-CoV-2, the virus that causes COVID-19, as well as other pathogens such as RSV and Influenza continues to provide vital information for interrupting chains of transmission and preventing or dampening the spread of new cases. Wastewater testing continues to gain traction as a viable means to monitor pathogens and provide timely information about the changing prevalence within individual communities. Wastewater-based epidemiology (WBE) utility has already been successfully described to track infectious diseases like hepatitis, norovirus, and polio. Viral shedding detected in wastewater often precedes case upticks in healthcare settings, providing valuable and rapid information about infection within a community. Multiple countries have implemented wastewater-based monitoring of SARS-CoV-2, and the COVID-19 case rates highly correlate with SARS-CoV-2 RNA found in wastewater. However, these upper-respiratory viruses are often found at low concentrations within wastewater, thereby necessitating a concentration step. Nanotrap Particles, coupled with GT Molecular kits, provide end users high-throughput and sensitive means to monitor pathogens such as SARS-CoV-2, Influenza, and RSV.