powerful tools for the capture and concentration of low abundance, low molecular weight peptides and proteins

0.7 μ) polyacrylamide microparticles (MPs) with internally-coupled Cibacron affinity dye demonstrate protective Our data support the mechanism of protection in which the immune defense induced by MPs along with the

Specifically, we have reported that exosomes transport viral proteins and RNA from infected cells to involved, we used siRNA to suppress expression of ESCRT (endosomal sorting complex required for transport) proteins

In the current manuscript, we examined the effect of Ebola structural proteins VP40, GP, NP and VLPs on recipient immune cells, as well as the effect of exosomes containing these proteins on naïve immune Exosome biogenesis was regulated by VP40 in transfected cells by increasing levels of ESCRT-II proteins EAP20 and EAP45, and exosomal marker proteins CD63 and Alix. Additionally, we utilized novel nanoparticles to safely capture VP40 and other viral proteins from Ebola

particles are hydrogel particles that are coupled to chemical dye affinity baits that bind a broad range of proteins Within minutes (<30 minutes), Nanotrap® particles concentrate low abundant proteins and viruses from

Packaging of cytokines and EBOV proteins into EVs from infected cells may be responsible for the decimation

are hydrogel nanoparticles functionalized with chemical affinity baits that can capture low abundance proteins Previously published studies have shown that Nanotraps can capture both virions and proteins from influenza that Nanotrap® sample processing increased assay sensitivity for EBOV-specific nucleic acid tests and protein

Frontiers in Microbiology, Feb 2016 Presence of Viral RNA and Proteins in Exosomes from Cellular Clones infected cells secrete exosomes that contain short viral RNAs, limited number of genomic RNAs, and viral proteins Viral proteins such as N and a modified form of NSs were present in some of these exosomes.

2015 Application of Nanotrap® technology for high sensitivity measurement of urinary outer surface protein analyte harvesting nanotechnology, Nanotrap® particles, we evaluated urinary Borrelia Outer surface protein

These vesicles contain proteins, RNA, and lipids from the cells they originate from and function in development Virally-infected cells can secrete viral as well as cellular proteins and RNA in exosomes, allowing viruses

POSTER Neurology, 2015 Detection of Human T-cell Lymphotropic Virus Type I proteins in exosomes from HAM/TSP patient CSF by novel Nanotrap® technology OBJECTIVE: To assess the presence of viral proteins The possibility of transfer of viral proteins via MV from viral reservoirs to uninfected cells in the HTLV-I viral proteins were assessed by Western blot analysis. CONCLUSIONS: These results suggest the possibility that HTLV-I protein present in virus-free CSF can

Previous studies demonstrated that NanoTrap® particles lead to both 100 percent capture of protein analytes