Changes in intracellular and EV-associated viral RNA were quantified using RT-qPCR, and changes in viral proteins , EV markers, and autophagy proteins were assessed by Western blot. Therefore, CBD may exert a protective effect by alleviating the pathogenic effects of EVs in HIV-1 and

using nanoparticle entrapment and mass spectrometry Presented at ASTMH 2018 Team detected parasite proteins Method also detected previously unrecognized proteins from multiple tick borne pathogens in urine samples Attributes of the technology include: 1) Rapid harvest of analytes, protection from degradation, and exclusion of unwanted high abundance host proteins; 2) Presence of unique chemical baits immobilized

We investigated the effect of cART (RT and protease inhibitors) on the content of extracellular vesicles

0.7 μ) polyacrylamide microparticles (MPs) with internally-coupled Cibacron affinity dye demonstrate protective Our data support the mechanism of protection in which the immune defense induced by MPs along with the

Frontiers in Microbiology, Feb 2016 Presence of Viral RNA and Proteins in Exosomes from Cellular Clones infected cells secrete exosomes that contain short viral RNAs, limited number of genomic RNAs, and viral proteins Viral proteins such as N and a modified form of NSs were present in some of these exosomes.

2015 Application of Nanotrap® technology for high sensitivity measurement of urinary outer surface protein analyte harvesting nanotechnology, Nanotrap® particles, we evaluated urinary Borrelia Outer surface protein

POSTER Neurology, 2015 Detection of Human T-cell Lymphotropic Virus Type I proteins in exosomes from HAM/TSP patient CSF by novel Nanotrap® technology OBJECTIVE: To assess the presence of viral proteins The possibility of transfer of viral proteins via MV from viral reservoirs to uninfected cells in the HTLV-I viral proteins were assessed by Western blot analysis. CONCLUSIONS: These results suggest the possibility that HTLV-I protein present in virus-free CSF can

developed a novel technology: Nanotrap® particles, which are designed to capture, concentrate, and protect demonstrating that Nanotrap® particles are able to capture live infectious virus, viral RNA, and viral proteins Capture is possible even in complex mixtures such as serum and allows the concentration and protection

PLoS ONE 2014 The Use of Nanotrap® Particles Technology in Capturing HIV-1 Virions and Viral Proteins This study demonstrates that majority of HIV-1 virions in culture supernatants and Tat/Nef proteins spiked Intracellular Nef protein was captured by NT080, while membrane-associated Nef was captured by NT086 and we propose to use this technology as a platform to enhance HIV-1 detection by concentrating viral proteins

Replication ​ The implementation of new antiretroviral therapies targeting transcription of early viral proteins Finally, we observed that neuronal cultures treated with Tat protein are protected from Tat-mediated

identification using shotgun proteomics approach Serum low-molecular weight (LMW) proteins potentially In this study three different methods, i.e. hydrogel nanoparticles, Proteominer(®) peptide ligand affinity A shotgun proteomics approach was adopted, with in-solution proteolytic digestion of the whole protein Data analysis, focusing on the LMW proteome (MW ≤ 40 kDa), has shown that the hydrogel nanoparticles for Proteominer(®) beads and Sartorius Vivaspin(®) device, respectively.

Journal of Proteome Research, May 29, 2012 Over the past few years, mass spectrometry has emerged as Application of mass spectrometry to protein and peptide measurement can provide advantages including Furthermore, an important caveat of SRM-based assays for proteins is that many low-abundance analytes reproducible with coefficients of variation of less than 30% for the measurement of important clinical proteins