Partner with Ceres to Elevate Your Discoveries Whether you’re building next-gen diagnostics, scaling surveillance, or running proteomics campaigns — Ceres is ready to help. Partner with us to accelerate your success. In this product portfolio brochure: Technology Overview Applications Where Nanotrap® Particle Technologies Drive Impact Infectious Disease Proteomics Liquid Biopsy Extracellular Vesicles DNA/RNA Extraction CeresNOW Technical Support BROCHURE Literature # GEN-BR31375

Mosquito-borne and waterborne pathogens such as arboviruses and Vibrio cholerae pose ongoing challenges for environmental and public health monitoring. Individual clinical testing is resource-intensive, logistically complex, and often limited by healthcare access or participation, resulting in an underestimation of asymptomatic infections.¹ Effective surveillance is essential for timely detection, outbreak prevention, and public health response. Wastewater-based epidemiology (WBE) has emerged as a promising approach for monitoring infectious diseases at the population level, yet its application to vector-borne pathogens remains underexplored. We evaluated Nanotrap® Microbiome Particles, magnetic hydrogel nanoparticles previously validated for capturing viruses and bacteria in environmental water and clinical specimens, for the capture and concentration of arboviruses and Vibrio cholerae from environmental water. POSTER SKU 44XXX Literature # WW-PO31485

Increasing interest in multi-omics profiling implicitly requires solutions that enable the analysis of the same biological sample by diverse techniques. Nanotrap® Particles provide complementary chemistries to selectively capture low-abundance analytes while removing high-abundance interferents in multiple contexts. Nanotrap® Protein Enrichment Affinity Kit (PEAK) enhances proteome depth by enriching low-abundance proteins and reducing albumin levels. Nanotrap® Extraction Advanced Technology (NEAT) Liquid Biopsy Kit isolates and concentrates cfDNA while minimizing genomic DNA contamination. Nanotrap® Extracellular Vesicle Particles enable effective enrichment of vesicle-associated biomarkers. The full suite of Nanotrap® Particle technology offers a flexible platform adaptable to multiple application areas across proteomics, genomics, extracellular vesicles (EVs), and infectious disease research. POSTER SKU # 34XXX Lit. # PL-PO31484

Nanotrap® Protein Enrichment Affinity Kit (PEAK) utilizes magnetic hydrogel particles to capture and concentrate low-abundance proteins and peptides, significantly improving protein identification in LC-MS/MS workflows. To enhance reproducibility and throughput, we integrated the Nanotrap® PEAK workflow onto the open-deck Opentrons FLEX® Proteomics Workstation, enabling scalable sample preparation across biofluids such as plasma and serum. The FLEX Proteomics Workstation is a modular, high-throughput liquid-handling robot designed to automate complex workflows through interchangeable pipettes, grippers, and labware. Integrating Nanotrap PEAK with this system minimizes manual handling, increases throughput, and delivers reproducible protein enrichment for up to 96 samples per run. POSTER SKU # 34XXX Lit. # PL-PO31483

Wastewater testing can be used to monitor SARS-CoV-2 infections in communities. Data from PCR-based wastewater testing are usually available to public health authorities within 5–7 days after excreta and other body fluids enter the sewer. While PCR-based methods can accurately detect and quantify SARS-CoV-2, sequencing-based methods are usually required to distinguish between variants, delaying the results and adding cost to the process. We developed and assessed a novel, customizable digital PCR (dPCR)-based genotyping method for SARS-CoV-2 variant detection in wastewater, which is more cost-effective, faster, and more accessible than sequencing. This approach was applied to more than 1,400 wastewater samples collected from six states between April 2023 and May 2024, with results displayed on a public dashboard alongside clinical data from the same period. The wastewater dPCR-based method effectively detected emerging variants, mirroring trends observed in clinical settings and in data on the Global Initiative on Sharing All Influenza Data (GISAID) platform; this method also provided early warning signals, as variants like EG.5 and FL were identified in wastewater before clinical detection. A subset of the wastewater samples was analyzed using both dPCR genotyping and sequencing, with good agreement between the two methods. The range of concordance for four different variants was between 62% and 98%. The successful development and implementation of this dPCR-based genotyping for wastewater samples demonstrates its cost-effectiveness and scalability. With the decline in clinical testing, wastewater surveillance becomes increasingly vital for monitoring SARS-CoV-2 variants and supplementing clinical surveillance efforts. ARTICLE: SKU 44XXX Mosavi SMR, Acer P, Andersen P, Barbero R, Barksdale S, Bellakbira S, Bunde D, Dunlap R, Erickson J, Goldfarb D, Jones-Roe T, Kilroy M, Le H, Lepene B, Milich E, Mohamed A, Munns D, Obermeyer J, Patnaik A, Pricer G, Reven MT, Richardson D, Ruhunusiri C, Sahoo SK, Saunders LP, Swahn O, Vengurlekar K, White D, Davis-Turak J, Stanz A, Flores-Baffi AP, Lozach J, Wesselman T, Hilton S, Kashwala S, Liu P, Moe CL, Sablon O, Wang Y, Wolfe M, Antkiewicz D, Camarato E, Janssen K, Roguet A, Shrestha S, Wied R, Gillespie J, Huang J, Jones A, Kane S, Gonzalez DS, Jarju ML, Lin C, Pascual ME, Poretsky R, Secreto M, Bradley I, Gallo S, Ye Y, Donahue E, Greenwald SM, Owens S, Wilton R. 0. A 1-year study on SARS-CoV-2 variant shifts in wastewater using dPCR: comparison with clinical and GISAID data. mSystems 0:e00229-25. https://doi.org/10.1128/msystems.00229-25

In this study, we evaluated three commercial cfDNA isolation kits to gauge their effectiveness in enriching and isolating cfDNA for colorectal cancer (CRC) detection via epigenetic screening. Methylation-specific real-time polymerase chain reaction (MSP RT-PCR) and high-resolution melting (HRM) results were used to compare the Nanotrap® Extraction Advanced Technology Liquid Biopsy Kit (NEAT Kit) to two other commercial kits for cfDNA extraction. Mock cfDNA samples were created using 1) standard methylated DNA from the HCT116 DKO cell line and 2) extracted DNA originating from colorectal cancer solid tumor resections; DNA was mechanically sheared to mimic the fragment size distribution of ctDNA. APPLICATION NOTE SKU 77XXX Lit # TS-AN31472

Vector-borne diseases, including yellow fever, Zika, chikungunya, West Nile, and dengue, are posing increasing health risks globally.(1) There is a need for a simple and effective workflow that can be used with wastewater samples to capture and concentrate multiple microbes from a single sample. This experiment demonstrates that the Nanotrap® Microbiome Workflow effectively captures and concentrates low-abundance, vector-borne disease microbes from a single wastewater sample. TECHNICAL NOTE: SKU 44XXX Literature #   WW-TN31413

Here we introduce a new sample preparation tool for plasma-based proteomics that is simple, easy-to-use, and versatile–Nanotrap® Protein Enrichment Affinity Kits. Nanotrap Protein Enrichment Affinity Kits use the Nanotrap magnetic hydrogel particle technology to capture and concentrate low abundance, low molecular weight proteins and peptides while simultaneously excluding higher molecular weight proteins. In this application note, we demonstrate how to use Nanotrap Protein Enrichment Kits to manually process plasma samples collected in K2EDTA blood collection tubes. We compare the number of unique protein identifications obtained from a plasma sample using different Nanotrap Protein Particle workflows, as compared to the same plasma sample processed without using the Nanotrap Protein Enrichment Kit. We also compare the overlap of unique protein identifications for these different workflows. APPLICATION NOTE SKU 34XXX Lit # PL-AN31387

Tuberculosis (TB) causes around 1.4 million deaths annually; earlier detection from complex samples like sputum can enable improved patient outcomes and treatment options.(1,2) Traditional methods like culture are slow and labor-intensive. While alternative molecular testing approaches like qPCR and sequencing are faster, they are hindered by inefficient sample preparation methods and the inhibitory nature of the sputum sample matrix. This study addresses these challenges by using Nanotrap® Microbiome B Particles to enhance Mycobacterium capture and concentration from sputum, offering a novel approach for TB detection. TECHNICAL NOTE: SKU 65XXX Literature #   WW-TN31420

To better understand and characterize the limitations of detection influenza A in wastewater samples, this study evaluated an enhanced workflow combining Nanotrap® Microbiome A Particles for viral enrichment, the Monarch® Mag Viral DNA/RNA Extraction kit with and without a DNase treatment step, and library preparation using the NEBNext® Flu A Integrated Indexing Primer Module protocol, followed by Oxford Nanopore Technologies sequencing. This protocol integrates targeted cDNA synthesis and native barcoding to enable same-day, whole-genome sequencing of influenza A viruses from complex wastewater matrices. The goal of this work was to assess genome coverage across several wastewater samples, evaluate the impact of DNase treatment on sequencing performance, and demonstrate a scalable, high-throughput solution for near real-time influenza surveillance. APPLICATION NOTE: SKU 44XXX Literature #   WW-AN31471

Wastewater surveillance is a critical tool for monitoring infectious diseases like COVID-19 at the community level.¹ As the virus evolves, with variants differing in transmissibility and virulence, tracking these changes is vital for public health. While quantitative PCR is the gold standard for detection and quantification, it typically can’t differentiate among many variants. In contrast, whole-genome sequencing (WGS) provides comprehensive data but is often too complex and resource-intensive for routine wastewater testing.² To address this, we developed a customizable digital PCR (dPCR)-based genotyping method for detecting SARS-CoV-2 variants in wastewater.³ This approach enables rapid, cost-effective screening for specific mutations, capturing both known and emerging variants. We compared dPCR genotyping to WGS using the same elution from each sample and applied the method to wastewater collected across Illinois (Oct 2023–Apr 2024). Results showed high concordance between the two methods. dPCR data aligned with clinical trends and detected early signals of variant rise and decline in near real-time, highlighting its potential as a scalable and timely tool for community-level variant tracking. POSTER: SKU 44XXX SKU 10XXX Literature # WW-PO31458

In this poster, see how the integration of the Nanotrap® Protein Enrichment Affinity Kit (PEAK), when paired with different digestion workflows, yielded substantial improvements in proteome depth compared to unenriched controls. Unique protein identifications using the Nanotrap PEAK workflow with each digestion kit—Pierce, PreOmics iST, and Thermo Scientific SMART Digest— show consistent improvement over neat plasma samples. Additionally, learn how the Gel-based evaluation via SYPRO Ruby staining confirmed the robust exclusion of the 65 kDa albumin band by Nanotrap® Protein A Particles while preserving a diverse range of protein profiles. POSTER: SKU 34XXX Literature #   PL-PO31473