Compatibility with a Downstream Epigenetic Workflow for Colorectal Cancer Detection

App Note -- Compatibility with a Downstream Epigenetic Workflow for Colorectal-PL-AN31472

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In this study, we evaluated three commercial cfDNA isolation kits to gauge their effectiveness in enriching

and isolating cfDNA for colorectal cancer (CRC) detection via epigenetic screening. Methylation-specific real-time

polymerase chain reaction (MSP RT-PCR) and high-resolution melting (HRM) results were used to compare

the Nanotrap® Extraction Advanced Technology Liquid Biopsy Kit (NEAT Kit) to two other commercial kits for

cfDNA extraction. Mock cfDNA samples were created using 1) standard methylated DNA from the HCT116 DKO

cell line and 2) extracted DNA originating from colorectal cancer solid tumor resections; DNA was mechanically

sheared to mimic the fragment size distribution of ctDNA.

APPLICATION NOTE SKU 77XXX

Lit # TS-AN31472