Plasma Proteomics Across Various Blood Collection Tubes Using Nanotrap PEAK

Different Blood Tubes-ASMS 2026-05272026 (1).pdf

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Pre-analytical factors — including blood-collection tube (BCT) additives, anticoagulant chemistry, and hemolysis state — influence plasma protein composition and affect downstream LC-MS/MS performance. Discovery proteomics workflows are typically validated on a single tube type, most commonly K2EDTA, and often require re-optimization or yield reduced proteome coverage when applied to alternative anticoagulants or hemolyzed specimens. Similar limitations arise when protein enrichment methods optimized for human plasma are applied to preclinical species.

We evaluated the Nanotrap® Protein Enrichment Affinity Kit (Nanotrap® PEAK) as a universal protein enrichment solution across multiple blood collection formats—including K2EDTA, K3EDTA, sodium citrate (NaCit), and hemolyzed samples—as well as non-human plasma from mouse and canine sources. The results highlight the flexibility of Nanotrap PEAK to support consistent protein enrichment across diverse matrices—helping enable more robust and scalable proteomics workflows.

Poster presented at the 2026 ASMS Conference.

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