Use of Nanotrap® Particles for Enrichment of Exosomes from Cell Culture Supernatant and CSF

POSTER


Presented at American Society for Exosomes and Microvesicles (ASEMV) 2018


This work demonstrates that Nanotrap® particles can be used to enrich exosomes from tissue culture supernatant and cerebrospinal fluid (CSF). Using tissue culture supernatants, Nanotrap® particle enrichment of exosomes was confirmed by Western Blot, CD81 ELISA, acetylcholinesterase (AchE) activity, and flow cytometry. Nanotrap® particle enrichment of exosomes from CSF and PBMCs were confirmed by CD81 ELISA and Western Blot. Finally, using tissue culture supernatants and a CD81 ELISA, it was demonstrated that Nanotrap® particles can be used to enrich exosomes to equal or greater concentrations than exosomes isolated by ultra centrifugation from 10 times the volume of the same starting material.


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