Use of Nanotrap® Particles for Enrichment of Exosomes from Cell Culture Supernatant and CSF

POSTER

Presented at American Society for Exosomes and Microvesicles (ASEMV) 2018

This work demonstrates that Nanotrap® particles can be used to enrich exosomes from tissue culture supernatant and cerebrospinal fluid (CSF). Using tissue culture supernatants, Nanotrap® particle enrichment of exosomes was confirmed by Western Blot, CD81 ELISA, acetylcholinesterase (AchE) activity, and flow cytometry. Nanotrap® particle enrichment of exosomes from CSF and PBMCs were confirmed by CD81 ELISA and Western Blot. Finally, using tissue culture supernatants and a CD81 ELISA, it was demonstrated that Nanotrap® particles can be used to enrich exosomes to equal or greater concentrations than exosomes isolated by ultra centrifugation from 10 times the volume of the same starting material.

Access full poster here