Reproducible, Deep Proteomic Profiling of Equine Synovial Fluid using NanotrapTechnology

SF-PO-31538_Reproducible, Deep Proteomic Profiling of Equine Synovial Fluid using Nanotrap.

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Synovial fluid proteomics presents analytical challenges similar to those encountered in plasma, including high dynamic range and the dominance of abundant proteins such as albumin and immunoglobulins, which can limit detection of lower-abundance, biologically relevant targets. These challenges are further compounded by variability in sample viscosity and handling conditions, creating a need for workflows that improve proteome depth while maintaining reproducibility and scalability.

The Nanotrap® Protein Enrichment Affinity Kit (PEAK) - Discovery uses magnetic hydrogel particles to enrich proteins from biofluids, improving access to lower-abundance regions of the proteome while remaining compatible with automated workflows, standard digestion protocols, and modern LC–MS/MS platforms. In this study, we evaluated the Nanotrap PEAK Discovery Method, which uses magnetic hydrogel particles to enrich low abundance proteins from biofluids while depleting high-abundance interferents.

Poster presented at the 2026 ASMS Conference.

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