Wastewater-based Epidemiology
Nanotrap® Microbiome Particles rapidly concentrate microbes from raw sewage, requiring no filtration, centrifugation, or bead-beating.
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Large-scale wastewater surveillance can be used to help communities monitor infection dynamics for SARS-CoV-2 and other microbes of concern. Robust, high-throughput methods based on Nanotrap Microbiome Particles are enabling wide-spread implementation.
A method using Nanotrap Microbiome A Particles was shown to detect 1 asymptomatic individual in a building of 415 residents and enabled early detection of nearly 85% of individual COVID-19 cases on a university campus.
Wastewater-based epidemiology workflow
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Simplified workflow.
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Eliminate filtration, centrifugation and bead beating methods, without sacrificing sensitivity.
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Decrease overall flume hood time
Highly compatible.
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Compatible with RT-qPCR, RT-ddPCR, RT-dPCR, and sequencing-based analysis methods.
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Manual and automated protocols available for use with a wide range of RNA partner extraction kits.
Capture more. Get better data.
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Improved capture efficiency with 2-10x higher recovery.
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Even better results – Add a Nanotrap Enhancement Reagent to improve results by another 1-2 Ct values.
Environmentally friendly.
Less plastics = Less waste.
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Decreased biohazard waste due to minimized plastics use compared to other methods.
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Increased available lab storage space.
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​Supported by the U.S. National Institutes of Health.
Webinars and Interviews
​Webinars
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Labroots Webinar: Deploying SARS-CoV-2 wastewater monitoring in Kansas. A case study and adoption of high-throughput, automated sample processing
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MACHEREY-NAGEL Webinar: Case Studies in Wastewater Surveillance - Successful Testing Methods in Practice
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Labroots Webinar: Sample collection and concentration methods -- impact on wastewater-based surveillance for multi-pathogen panels
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Interviews
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Interview with Nevada State Public Health Laboratory: How Nanotrap® Particle Technology is Improving the Public Health Response to SARS-CoV-2
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Interview with University of Massachusetts, Amherst: How Nanotrap® Technology for Wastewater Surveillance Improves SARS-CoV-2 Workflows
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Poster & applications related to wastewater-based epidemiology testing
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Poster: Surveillance of SARS-CoV-2 in wastewater of Ohio: Consolidation of SARS-CoV-2 testing from academic, commercial and government labs to Ohio Department of Health Laboratory
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Application Notes
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Capture and Concentrate Microbes that Cause Sexually Transmitted Infections from Wastewater
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Capture and Concentrate Pathogenic Legionella Species from Environmental Water Samples
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Nanotrap Microbiome A and B Particles can be combined to improve microbe capture from wastewater
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Wastewater sequencing uncovers early, cryptic SARS-CoV-2 variant transmission
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Comparison of Nanotrap Microbiome A Particles, membrane filtration, and skim milk workflows for SARS-CoV-2 concentration in wastewater