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Wastewater-Based Epidemiology

Nanotrap® Microbiome Particles rapidly concentrate microbes from raw sewage, requiring no filtration, centrifugation, or bead-beating.

Large-scale wastewater surveillance can be used to help communities monitor infection dynamics for SARS-CoV-2 and other microbes of concern. Robust, high-throughput methods based on Nanotrap Microbiome Particles are enabling wide-spread implementation.



Nanotrap® Microbiome Particles enable rapid and simple automated or manual methods for preparing wastewater samples for nucleic acid extraction.

Replace filtration, centrifugation, and bead-beating methods, without sacrificing assay sensitivity.

Add a Nanotrap® Enhancement Reagent to improve results by another 1-2 Ct values.

Nanotrap Microbiome A Particles and Nanotrap Microbiome B Particles can be combined to improve microbe capture from wastewater.

An automated method using Nanotrap Microbiome Particles can concentrate microbes from 24 wastewater samples in a 40-minute run, offering substantial throughput improvements.

Compatible with RT-qPCR, RT-ddPCR, RT-dPCR, and sequencing based analysis methods.

Manual and automated protocols available for use with a wide range of RNA extraction kits from partners.

Supported by the U.S. National Institutes of Health.

A method using Nanotrap Microbiome A Particles was shown to detect 1 asymptomatic individual in a building of 415 residents and enabled early detection of nearly 85% of individual COVID-19 cases on a university campus.

Nanotrap particles, PEG, HA Filter comparison SK et al 2021.png
Nanotrap Microbiome A Particle method compared to PEG precipitation and HA filtration methods. Data source: Karthikeyan S, et al. mSystems 2021. DOI: 10.1128/mSystems.00045-21
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