Nanotrap® Microbiome A and B Particles can be Combined to Improve Microbe Capture from Wastewater

Updated: Nov 18


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Key Advantages

> Nanotrap® Microbiome A Particles and Nanotrap Microbiome B Particles can be used in a single method to rapidly capture and concentrate bacterial and viral targets, including: Campylobacter jejuni, Escherichia coli, Listeria monocytogenes, Clostridium difficile, Salmonella enterica subsp. enterica, SARS-CoV-2, norovirus GII virus, influenza A virus, influenza B virus, and respiratory syncytial virus (RSV) from wastewater without the need of filtration and centrifugation.

> The combined workflow can be automated on a Kingfisher Apex System.

> Wastewater samples processed using Nanotrap Microbiome A Particles and Nanotrap Microbiome B Particles are compatible with digital PCR (dPCR) systems like the QIAcuity One 5plex.


Wastewater-based epidemiology has become widely recognized as a powerful tool to identify and monitor emerg-ing pathogens in a community. Many of the efforts to date have been focused on developing wastewater testing methods that can enable monitoring of SARS-CoV-2 using RT-qPCR, but there is growing interest in expanding to other microbes and to other detection methods, like dPCR.

Nanotrap Microbiome Particles enable rapid concentration of microbes from raw sewage, requiring no filtration or centrifugation and is compatible with RT-qPCR, RT-ddPCR, RT-dPCR, and sequencing based analysis methods.

Labs around the world have used Nanotrap Microbiome A Particles to process many tens of thousands of wastewater samples for detection of SARS-CoV-2. Moreover, it was recently demonstrated that Nanotrap particle processing of wastewater testing can enable detection of emerging variants of concern up to 14 days earlier than clinical genomic surveillance. Nanotrap particle methods have also enabled detection of monkeypox and hepatitis A virus in wastewater.

Here, we introduce a new method from Ceres Nanosciences: combining Nanotrap Microbiome B Particles with Nanotrap Microbiome A Particles in a single workflow to improve and expand microbe detection in wastewater samples. This simple and sensitive method is compatible with multiple nucleic acid extraction kits.

In this application note, we show that Nanotrap Microbiome A Particles and Nanotrap Microbiome B Particles can be combined in a single workflow: 1) to capture and concentrate multiple pathogenic bacteria and viruses from wastewater samples; 2) to improve detection of those bacteria and viruses relative to using Nanotrap Microbiome A Particles alone; and 3) that is compatible with dPCR assays run on the QIAcuity One 5plex.

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