One of the challenges of working with cell-free DNA (cfDNA) is its small size. cfDNA fragments tend to be 167 bp or multiples thereof, and traditional bead-based DNA extraction methods are less efficient at capturing small-size DNA. Traditional DNA extraction methods have a lower fragment size cutoff of ~50 bp, and the yield is typically lower as the fragment size gets closer to 50 bp. This study will compare the recovery of different extraction methods in this size range and

Liquid biopsy represents a highly sensitive, non-invasive approach to detect cancer biomarkers early and monitor response to treatments....

Next-generation sequencing (NGS) is often used in liquid biopsy research to detect a wide range of genes or whole genome sequencing....

Presently, both DNA and RNA commercial extraction kits use a common method in which a crowding agent or alcohol induces a conformational...