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App Note: Promega - Viral Concentration and Viral RNA Purification from Wastewater

Viral Concentration and Viral RNA Purification from Wastewater on the KingFisher™ Flex Purification System


Key Advantages

Monitoring SARS-CoV-2 viral RNA in wastewater can be used to detect and predict COVID-19 disease outbreaks. Here, we describe an automated high-throughput protocol for viral concentration and viral RNA purification from wastewater for SARS-CoV-2 detection.


Early in the COVID-19 pandemic, studies showed that SARS-CoV-2 viral RNA could be detected in the feces of infected individuals. Detection of SARS-CoV-2 in wastewater can indicate the presence or prevalence of COVID-19 within a community. Routine testing of large numbers of wastewater samples is important to obtain sufficient and timely data on viral spread or emergence of new variants.

To address the need for high-throughput wastewater surveillance workflows, we developed the Maxwell® HT Environmental TNA Kit, which allows automated high-throughput purification of viral RNA. The following protocol uses Nanotrap® Magnetic Virus Particles for automated concentration of SARS-CoV-2 and PMMoV from wastewater samples, then purification of viral RNA using the Maxwell® HT Environmental TNA Kit on a KingFisher™ Flex Purification System.


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