Balancing Proteome Depth, Reproducibility, and Cost in Plasma Proteomics

Poster -- Balancing Proteome Depth Reproducibility and Cost in Plasma Proteomics - PL-PO31.

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INTRODUCTION

Plasma proteomics is inherently challenging due to its extreme dynamic range, with a small number of highly abundant proteins dominating the sample and limiting detection of lower-abundance, biologically relevant proteins. Enrichment strategies are therefore needed to increase proteome depth without introducing excessive variability, cost, or workflow complexity. The Nanotrap® Protein Enrichment Affinity Kit (PEAK) - Discovery uses magnetic hydrogel particles to selectively enrich proteins from plasma, improving access to low-abundance regions of the proteome while remaining

compatible with standard digestion workflows and modern LC–MS/MS platforms.

In this study, we evaluated the Nanotrap® PEAK Discovery workflow for proteome depth and reproducibility in human

plasma, compared with unenriched samples and alternative commercial enrichment kits, using a standardized digestion protocol and dia-PASEF® analysis on a Bruker timsTOF HT, with a focus on depth, reproducibility, and cost efficiency.

Poster presented at the US HUPO 2026 Conference.

POSTER SKU 34XXX

Literature # PL-PO31526