medRxiv May 2023 Background The origin of divergent SARS-CoV-2 spike sequences found in wastewater, but These “cryptic” wastewater sequences have harbored many of the same mutations that later emerged in Omicron We first detected a cryptic lineage in municipal wastewater in Wisconsin in January 2022. Findings The persistence of the Wisconsin Lineage signal allowed us to trace it from a central wastewater Interpretation We propose that prolonged detection of the Wisconsin Lineage in wastewater represents

Wastewater surveillance has become a vital tool for monitoring infectious diseases like COVID-19 at the virulence, tracking the emergence and prevalence of these variants has become a critical component of wastewater-based sequencing—while comprehensive—is often too complex, time-consuming, and resource-intensive for many wastewater developed a customizable digital PCR (dPCR)-based genotyping approach to detect SARS-CoV-2 variants in wastewater from a year-long surveillance effort across Georgia (April 2023–April 2024) highlight the potential of wastewater-based

"Intensity of sample processing methods impacts wastewater SARS-CoV-2 whole genome amplicon sequencing outcomes" Science of The Total Environment, June 2023 ABSTRACT: Wastewater SARS-CoV-2 surveillance has Genomic surveillance of SARS-CoV-2 in wastewater, particularly the efforts for whole genome sequencing The intrinsic sample limitations are inherent to wastewater. samples, played important roles in wastewater SARS-CoV-2 amplicon sequencing.

bioRxiv, February 2022 ABSTRACT: Monitoring wastewater samples at building-level resolution screens large Here we perform untargeted metatranscriptomics on virally-enriched wastewater samples from 10 locations emergent threats through changes in the human microbiome, suggesting new approaches for untargeted wastewater-based

International Airport and explores the utility of a dual-sample strategy leveraging environmental aircraft wastewater Methods: Using a combined pooled nasal swab and aircraft wastewater sampling approach resulted in complementary Results: Mutational profiling in source pooled nasal swabs and aircraft wastewater sample data revealed Muvunyi, Early detection of SARS-CoV-2 variants using genomic surveillance: insights from aircraft wastewater

SARS-CoV-2 RNA concentration in wastewater successfully tracks regional infection dynamics and provides Tracking virus genomic sequences in wastewater would improve community prevalence estimates and detect However, two factors limit wastewater-based genomic surveillance: low-quality sequence data and inability Here, we resolve these critical issues to perform a high-resolution, 295-day wastewater and clinical Our study provides a scalable solution for wastewater genomic surveillance that allows early detection

Morbidity and Mortality Weekly Report, February 2023 Notes from the Field: Aircraft Wastewater Surveillance Because SARS-CoV-2 can be detected in feces and urine of some infected persons (2), wastewater surveillance Sampling wastewater directly from aircraft can be used to link SARS-CoV-2 lineage data with flight origin

Enterica, and crAssphage from wastewater without the need of filtration, bead-beating, or centrifugation Introduction Recently, wastewater surveillance has become widely recognized as a powerful tool to identify Many of the investments made to date have been focused on developing wastewater surveillance methods Moreover, it was recently demonstrated that Nanotrap particle processing of wastewater testing can enable Nanotrap particle methods have also enabled detection of monkeypox and hepatitis A virus in wastewater

"Wastewater Surveillance of U.S. A pilot study of wastewater testing was initiated in February, 2021 to determine its feasibility as a Wastewater was analyzed for the presence of two viral genes, N and E, and quantified relative to levels Wastewater data was validated by comparison with concomitant screening and surveillance programs that strategic locations and is actively continuing its wastewater testing program.

In this application note, we show that Nanotrap® Microbiome A Particles with the MagMAX™ Wastewater Ultra Nucleic Acid Isolation Kit is compatible with the Clear Dx™ FlexPro: Wastewater (SARS-CoV-2) and leads Wastewater-based epidemiology (WBE) increased in prevalence during the pandemic as a way to measure disease Accurate and complete sequencing from wastewater requires clean and concentrated nucleic acid samples The Clear Dx FlexPro: Wastewater (SARS-CoV-2) WGS Reagent and Automation Bundle uses targeted sequencing

"Wastewater-Based Epidemiology for COVID-19: Handling qPCR Nondetects and Comparing Spatially Granular Wastewater and Clinical Data Trends" ACS EST Water July, 2022 ABSTRACT: Wastewater-based epidemiology While community-scale wastewater and clinical data frequently correlate, less is known about subcommunity Moreover, nondetects in qPCR wastewater data are typically handled through methods known to bias results We also present a novel framework for spatially aligning clinical data with wastewater data collected

norovirus GII virus, influenza A virus, influenza B virus, and respiratory syncytial virus (RSV) from wastewater Introduction Wastewater-based epidemiology has become widely recognized as a powerful tool to identify Many of the efforts to date have been focused on developing wastewater testing methods that can enable Moreover, it was recently demonstrated that Nanotrap particle processing of wastewater testing can enable Nanotrap particle methods have also enabled detection of monkeypox and hepatitis A virus in wastewater