APPLICATION NOTE SKU 10XXX SKU 65XXX
Key Advantages
> Nanotrap® Microbiome B Particles rapidly capture and concentrate Campylobacter jejuni, Escherichia coli O157:H7, Listeria monocytogenes, Clostridium difficile, Salmonella enterica subsp. Enterica, and crAssphage from wastewater without the need of filtration, bead-beating, or centrifugation.
> Nanotrap Microbiome B Particles enable a simple and rapid automated or manual method for detecting pathogens, including bacteria, in wastewater samples.
> Nanotrap Microbiome B Particles are compatible with several commercially available nucleic acid extraction kits.
Introduction
Recently, wastewater surveillance has become widely recognized as a powerful tool to identify and monitor emerging pathogens in a community. Many of the investments made to date have been focused on developing wastewater surveillance methods that can enable rapid and reliable monitoring of SARS-CoV-2, but there is growing interest in expanding the utility of these methods to other microbes.
The Nanotrap particle technology enables rapid concentration of microbes from raw sewage, requiring no filtration or centrifugation and is compatible with RT-qPCR, RT-ddPCR, RT-dPCR, and sequencing based analysis methods. Labs around the world have used Nanotrap Microbiome A Particles to process many tens of thousands of wastewater samples for detection of SARS-CoV-2. Moreover, it was recently demonstrated that Nanotrap particle processing of wastewater testing can enable detection of emerging variants of concern up to 14 days earlier than clinical genomic
Surveillance. Nanotrap particle methods have also enabled detection of monkeypox and hepatitis A virus in wastewater.
Here, we introduce two new products from Ceres Nanosciences: Nanotrap Microbiome B Particles and Nanotrap Enhancement Reagent 3 (ER3), along with a sensitive, rapid, and easy-to-use concentration method for wastewater-based epidemiology testing for bacterial targets. This simple and sensitive method is compatible with several magnetic bead DNA extraction kits including standard “off the shelf” kits provided by MACHEREY-NAGEL and Thermo Fisher Scientific and requires no bead-beating steps.
We show that Nanotrap Microbiome B Particles 1) capture and concentrate multiple pathogenic bacteria from wastewater samples resulting in equivalent or better detection as compared to labor-intensive manual HA Filter wastewater concentration methods, 2) are compatible with multiple automation-friendly nucleic acid extraction kits, and 3) enable detection of a wastewater control organism that is commonly utilized to normalize detection results across unique wastewater samples.
