Wastewater-based surveillance of respiratory pathogens is challenging due to low target abundance and complex sample matrices. This study demonstrates that Nanotrap® Microbiome Particles can enhance native pathogen recovery and detection sensitivity from influent wastewater, enabling more comprehensive monitoring of respiratory disease circulation in communities. In This Experiment Recovery and sensitivity using the Nanotrap® Microbiome Workflows were evaluated relative to co

Nanotrap® Microbiome Particles are widely used in wastewater surveillance to capture and concentrate microbial pathogens, enhancing the sensitivity and reliability of pathogen detection. This approach enables effective monitoring of microbes, providing valuable insights for public health initiatives. Following the concentration step, efficient nucleic acid extraction is critical to ensure the recovery of high-quality RNA and DNA for downstream molecular applications. The MagM

One of the challenges of working with cell-free DNA (cfDNA) is its small size. cfDNA fragments tend to be 167 bp or multiples thereof, and traditional bead-based DNA extraction methods are less efficient at capturing small-size DNA. Traditional DNA extraction methods have a lower fragment size cutoff of ~50 bp, and the yield is typically lower as the fragment size gets closer to 50 bp. This study will compare the recovery of different extraction methods in this size range and