Wastewater testing can be used to monitor SARS-CoV-2 infections in communities. Data from PCR-based wastewater testing are usually available to public health authorities within 5–7 days after excreta and other body fluids enter the sewer. While PCR-based methods can accurately detect and quantify SARS-CoV-2, sequencing-based methods are usually required to distinguish between variants, delaying the results and adding cost to the process. We developed and assessed a novel, cus

In this study, we evaluated three commercial cfDNA isolation kits to gauge their effectiveness in enriching and isolating cfDNA for colorectal cancer (CRC) detection via epigenetic screening. Methylation-specific real-time polymerase chain reaction (MSP RT-PCR) and high-resolution melting (HRM) results were used to compare the Nanotrap® Extraction Advanced Technology Liquid Biopsy Kit (NEAT Kit) to two other commercial kits for cfDNA extraction. Mock cfDNA samples were create

Vector-borne diseases, including yellow fever, Zika, chikungunya, West Nile, and dengue, are posing increasing health risks globally.(1) There is a need for a simple and effective workflow that can be used with wastewater samples to capture and concentrate multiple microbes from a single sample. This experiment demonstrates that the Nanotrap® Microbiome Workflow effectively captures and concentrates low-abundance, vector-borne disease microbes from a single wastewater sample.