A Sample-to-Results Workflow for Influenza A Sequencing from Wastewater
- Caryn Tyler
- Sep 25
- 1 min read
To better understand and characterize the limitations of detection influenza A in wastewater samples, this study evaluated an enhanced workflow combining Nanotrap® Microbiome A Particles for viral enrichment, the Monarch® Mag Viral DNA/RNA Extraction kit with and without a DNase treatment step, and library preparation using the NEBNext® Flu A Integrated Indexing Primer Module protocol, followed by Oxford Nanopore Technologies sequencing. This protocol integrates targeted cDNA synthesis and native barcoding to enable same-day, whole-genome sequencing of influenza A viruses from complex wastewater matrices.
The goal of this work was to assess genome coverage across several wastewater samples, evaluate the impact of DNase treatment on sequencing performance, and demonstrate a scalable, high-throughput solution for near real-time
influenza surveillance.
APPLICATION NOTE: SKU 44XXX
Literature # WW-AN31471