The purpose of this study was to demonstrate that the NEAT Liquid Biopsy Kit-Streck BCT is suitable for use with the Streck Cell-Free DNA BCT®, effectively capturing and concentrating cell-free DNA (cfDNA) under varied storage conditions specified by the manufacturer. The data underscore the superior performance of the NEAT Kit compared to a competitor, Kit 2, in the cfDNA capture and concentration. These findings confirm that the NEAT Liquid Biopsy Kit is a dependable and efficient method
for cfDNA capture and concentration.
Blood samples from three donors were drawn into Streck Cell- Free DNA BCT. The tubes were stored at room temperature (19°C-23°C). After days two and seven, plasma was collected per tube manufacturer’s instructions. cfDNA was isolated from 4 mL of plasma sample using competitor Kit 2 (automated, magnetic silica bead-based method) and the NEAT Liquid Biopsy Kit - Streck BCT. Samples were processed using the KingFisher™ Apex System. cfDNA was analyzed using the Agilent Cell-Free DNA ScreenTape on the 4200 TapeStation. Bars represent the concentrations of the average of three technical replicates and error bars indicate one standard deviation.
TECHNICAL NOTE SKU 10XXX SKU 55XXX