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Protocol for isolation of Extracellular Vesicles using Nanotrap particles

Journal of Visual Experiments (JoVE): Purification of High Yield Extracellular Vesicle Preparations Away from Virus


This protocol isolates extracellular vesicles (EVs) away from virions with high efficiency and yield by incorporating EV precipitation, density gradient ultracentrifugation, and particle capture to allow for a streamlined workflow and a reduction of starting volume requirements, resulting in reproducible preparations for use in all EV research.



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