SARS-CoV-2 extraction from wastewater concentrated with Nanotrap® Magnetic Virus Particles


APPLICATION NOTE SKU 44XXX


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MACHERY NAGEL: SARS-CoV-2 extraction from wastewater concentrated with Nanotrap® Magnetic Virus Particles


Automatable concentration and purification of viral RNA from wastewater


Introduction


Many researchers, private companies, and public health agencies are utilizing wastewater testing to monitor COVID-19 infections in selected communities, campus dormitories, or health centers. Wastewater surveillance efforts can be used to look for early warning signs of an upcoming rise in infection rates, and the methods employed by these researchers can be used to estimate numbers of infected individuals who may or may not be symptomatic. Routine wastewater surveillance may prove to be an efficient, non-invasive tool to detect not only COVID-19 infections due to the SARS-CoV-2 virus, but other types of future viral outbreaks as well.


Viral RNA extraction from wastewater is a major challenge for laboratories due to the presence of so many substances that cause inhibition of downstream analysis, and because large volumes of wastewater are often needed for detecting the presence of the SARS-CoV-2 virus. Commonly used wastewater concentration methods tend to exacerbate the problem since they also usually concentrate the inhibitors as well. Many concentration techniques are also labor intensive and costly. In this application note we show an efficient, cost-effective, and even automatable method to extract SARS-CoV-2 viral RNA from complex wastewater samples by pairing MACHEREY‑NAGEL’s NucleoMag® DNA/RNA Water kit for extraction with the Nanotrap® Magnetic Virus Particles from Ceres Nanosciences for wastewater concentration





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