INTRODUCTION Plasma proteomics is inherently challenging due to its extreme dynamic range, with a small biologically relevant proteins. Enrichment strategies are therefore needed to increase proteome depth without introducing excessive variability selectively enrich proteins from plasma, improving access to low-abundance regions of the proteome while In this study, we evaluated the Nanotrap® PEAK Discovery workflow for proteome depth and reproducibility

Plasma proteomics workflows increasingly demand lower sample input — for biobanked or pediatric specimens Scaling the workflow down without sacrificing proteome depth would broaden access to low-input matrices Both workflows use identical chemistries — Nanotrap® Protein A, Nanotrap® Protein B, and Nanotrap® Protein We assess protein-group identifications, proteome overlap, rank-abundance distributions, and cytokine detection to assess the trade-off between sample input and proteome depth.

The UC Davis Proteomics Core lab offers state-of-the-art LC-MS/MS analysis, including protein identification , proteomics profiling, targeted proteomics, and post-translational modification analysis, for both on

Quantitative and discovery plasma proteomics requires workflows that maximize protein identifications The Nanotrap® Protein Enrichment Affinity Discovery Kit (Nanotrap® Discovery Kit) delivers highly reproducible , deep proteome coverage while reducing per-sample cost compared to other enrichment kits. In This Experiment We evaluated whether the manual Nanotrap Discovery Kit enhances proteome coverage

Synovial fluid proteomics presents analytical challenges similar to those encountered in plasma, including high dynamic range and the dominance of abundant proteins such as albumin and immunoglobulins, which variability in sample viscosity and handling conditions, creating a need for workflows that improve proteome The Nanotrap® Protein Enrichment Affinity Kit (PEAK) - Discovery uses magnetic hydrogel particles to enrich proteins from biofluids, improving access to lower-abundance regions of the proteome while remaining

blood-collection tube (BCT) additives, anticoagulant chemistry, and hemolysis state — influence plasma protein Discovery proteomics workflows are typically validated on a single tube type, most commonly K2EDTA, and often require re-optimization or yield reduced proteome coverage when applied to alternative anticoagulants We evaluated the Nanotrap® Protein Enrichment Affinity Kit (Nanotrap® PEAK) as a universal protein enrichment diverse matrices—helping enable more robust and scalable proteomics workflows.

In proteomics, achieving high reproducibility and deep proteome coverage is essential for confident biomarker assessing reproducibility is the coefficient of variation (CV), which quantifies the consistency of protein )—applied through both manual and semi-automated protocols—enables substantial improvements in both proteome neat plasma processing, the Nanotrap PEAK manual and KingFisher™ Apex System protocols reduced median protein results establish Nanotrap PEAK workflows as highly efficient and reproducible solutions for plasma proteomics

Maximizing the interpretive power and utility of proteomic data for Clinical and Precision Health research Discovery based workflows that yield comprehensive proteomes with high analytical precision (low assay apply to Plasma as well as to Dried Blood, in which an inherently more complex matrix often constrains proteome Finally, we preliminarily evaluated our method for utility for routine, cost effective discovery-based proteomic and pathway-level signal from VDBS — establishing a scalable, high-throughput platform for clinical proteomics

Nanotrap® Protein Enrichment Affinity Kit (PEAK) utilizes magnetic hydrogel particles to capture and concentrate low-abundance proteins and peptides, significantly improving protein identification in LC-MS reproducibility and throughput, we integrated the Nanotrap® PEAK workflow onto the open-deck Opentrons FLEX® Proteomics The FLEX Proteomics Workstation is a modular, high-throughput liquid-handling robot designed to automate Nanotrap PEAK with this system minimizes manual handling, increases throughput, and delivers reproducible protein

In this poster, see how the integration of the Nanotrap® Protein Enrichment Affinity Kit (PEAK), when paired with different digestion workflows, yielded substantial improvements in proteome depth compared Unique protein identifications using the Nanotrap PEAK workflow with each digestion kit—Pierce, PreOmics evaluation via SYPRO Ruby staining confirmed the robust exclusion of the 65 kDa albumin band by Nanotrap® Protein A Particles while preserving a diverse range of protein profiles.

Nanotrap® Protein Enrichment Affinity Kit (PEAK) enhances proteome depth by enriching low-abundance proteins Nanotrap® Particle technology offers a flexible platform adaptable to multiple application areas across proteomics

In this study, we demonstrate that protein enrichment from human plasma using the Nanotrap® Protein Enrichment