Sample Miniaturization for Plasma Proteomics Using Nanotrap PEAK

ASMS 2026 - Sample_Miniaturization_Poster_05272026.pdf

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Plasma proteomics workflows increasingly demand lower sample input — for biobanked or pediatric specimens where volume is constrained, and for high-throughput screening where reagent cost per sample drives project feasibility. The Nanotrap® Protein Enrichment Affinity Kit (Nanotrap® PEAK) is validated at a 50 μL plasma input, where magnetic hydrogel particles functionalized with complementary affinity baits enrich low-abundance proteins and deplete high-abundance interferents prior to LC-MS/MS. Scaling the workflow down without sacrificing proteome depth would broaden access to low-input matrices and reduce per-sample cost.

We evaluated a 10 μL Nanotrap® PEAK Discovery Method against the standard 50 μL workflow on matched K2EDTA plasma samples. The 10 μL method reduces particle consumption by 75% and lowers reagent cost per sample by ~75%. Both workflows use identical chemistries — Nanotrap® Protein A, Nanotrap® Protein B, and Nanotrap® Protein C Particles — and the same downstream digestion and LC-MS/MS pipeline. We assess protein-group identifications, proteome overlap, rank-abundance distributions, and cytokine detection to assess the trade-off between sample input and proteome depth.

Poster presented at the 2026 ASMS Conference.

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