Improving RNA extraction for a more sensitive qRT-PCR assay
APPLICATION NOTE SKU 44XXX
Key Advantages
> Circumvent the low input sample volume restrictions of most extraction methods by capturing influenza A from larger volumes.
> Capture and concentrate influenza virus from transport media with Nanotrap particles for greater viral RNA extraction yield and improved qRT-PCR assay sensitivity.
Introduction
Influenza is responsible for seasonal epidemics each year in the United States that result in an average of 200,000 hospitalizations and tens of thousands of deaths. Accurate and early diagnosis of influenza viral infections are critical for effective treatment and limiting the spread of disease.
Nucleic acid-based tests demonstrate high sensitivity and specificity as compared to antigen-based tests; however, limitations on input sample volume can still present sensitivity challenges in complex sample types. Using a simple and efficient pre-concentration workflow in combination with existing molecular test formats can improve detection at earlier time points following infection.
Nanotrap Virus Particles can capture, concentrate, and preserve viral pathogens from biological samples and improve downstream diagnostic assays such as enzyme-linked immunosorbent assays (ELISA), next generation sequencing assays (NGS), lateral flow assays (LFA), plaque infectivity assays, and quantitative reverse-transcription polymerase chain reaction assays (qRT-PCR).
Here we demonstrate the impact of Nanotrap particle sample processing on influenza virus RNA extraction from viral transport media with QIAGEN’s QIAamp® Viral RNA Mini kit, followed by qRT-PCR detection.