App Note: Nanotrap® Magnetic Virus Particles Enable Sequencing in Wastewater


APPLICATION NOTE SKU 10XXX SKU 44XXX


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Key Advantages


> Nanotrap® Magnetic Virus Particles improve wastewater sequencing workflows and improve viral pathogen sequencing results.

> Enables sequencing of multiple SARS-CoV-2 variants.


> Enables wastewater surveillance of multiple virus types from a single sample, including SARS-CoV-2 and Influenza A.


Introduction


The continuing emergence of novel SARS-CoV-2 variants and other problematic viral species has highlighted the need for accurate, rapid, and deployable sequencing methods. Detection of these viruses through the collection and analysis of municipal wastewater sources is a rapidly evolving detection strategy that has growing utility in the health response to the ongoing SARS-CoV-2 pandemic. Effective sequencing from wastewater typically requires a concentration step or enrichment strategy to alleviate the issues caused by low viral titers and enzyme inhibition inherent to these wastewater samples. Commonly these methods, such as HA filtration, are time consuming and arduous, needlessly limiting the analytical utility of monitoring wastewater.


Nanotrap® Magnetic Virus Particles enable a wastewater enrichment strategy that is sensitive, rapid, easy to use, and can be performed manually or in an automated setup. Because it requires no filtration or centrifugation steps, this approach is compatible with medium- and high-throughput settings. Demonstrated previously, Nanotrap® Magnetic Virus Particles are able to capture and concentrate multiple strains of coronavirus (along with other viruses such as Influenza), thus enhancing detection of those viruses, even in co-infection scenarios.


Nanotrap® Magnetic Virus Particles can enable automated viral surveillance of wastewater using a standard magnetic bead-based RNA extraction method. Coupled with the Oxford Nanopore Technologies™ MinION™ sequencing platform, Nanotrap® Magnetic Virus Particles increase total viral mapped reads, resulting in greater sequencing depth and total viral coverage. Here, we demonstrate that the Nanotrap® Magnetic Virus Particles workflow can: 1) significantly improve sequencing of low viral titer SARS-CoV-2 positive wastewater samples relative to a standard HA Filter Concentration Method, 2) enable sequencing of multiple SARS-CoV-2 variants, and 3) enrich multiple viral types, including Influenza A, from a single sample.







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