App Note: Nanotrap® Particles with RT‐ddPCR Assay for SARS‐CoV‐2 Variant Detection in Wastewater


APPLICATION NOTE SKU 10XXX SKU 44XXX


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Key Advantages


> Enables detection of multiple SARS‐CoV‐2 variants


> Provides absolute quantification of low copy number samples with higher precision


Introduction


Since the emergence of new SARS-CoV-2 variants, which have been associated with increased transmissibility and/or immune escape, there is an urgent need for methods that enable specific and timely detection and quantification of the occurrence of these variants in the community.

Droplet Digital Polymerase Chain Reaction (ddPCR™) was developed to provide high-precision, absolute quantification of nucleic acid target sequences within samples through sample partitioning. This technology also mitigates the effects of target competition, making PCR amplification less sensitive to inhibition.


In this application note, we show the compatibility of Nanotrap® Magnetic Virus Particles with RT-ddPCR and demonstrate the detection and absolute quantification of SARS-CoV-2 variants (i.e. Omicron, Delta) in wastewater samples.







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