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App Note: Rapid SARS-CoV-2 Viral Isolation from Wastewater

Updated: Dec 7, 2020


Key Advantages

> Nanotrap® Magnetic Virus Particles concentrate SARS-CoV-2 wastewater sample with no centrifugation or filtration.


According to the Centers for Disease Control and Prevention, SARS-CoV-2 can be shed in the feces of individuals with asymptomatic or symptomatic infections, making SARS-CoV-2 RNA detection in sewage a COVID-19 indicator that is independent of healthcare-seeking behaviors and access to clinical testing.1 Due to low concentrations in water samples such as sewage, SARS-CoV-2 viral particles must be concentrated prior to nucleic acid isolation. The three most common approaches for viral particle concentration are filtration and concentration using electronegative membranes, ultrafiltration, and precipitation. These approaches are time consuming and cumbersome, requiring access to complex laboratory equipment like centrifuges, which limits the daily sample throughput. 2-5

Here we describe a simple and rapid approach, using Nanotrap® Magnetic Virus Particles to capture and concentrate SARS-CoV2 from wastewater samples prior to nucleic acid isolation. Because it requires no filtration or centrifugation steps, this approach is amenable to high throughput implementation.


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