Detection of Monkeypox Virus in Wastewater - Use of Nanotrap® Particles


APPLICATION NOTE SKU 10XXX SKU 44XXX


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Key Advantages


> A high-throughput method to capture and concentrate multiple pathogens including Monkeypox virus


> Scalable and automatable method with increased sensitivity using large volume samples


> Compatible with a variety of extraction kits and downstream methods


> Utilizes the same Nanotrap® particle-enabled work-flow as SARS-CoV-2


Introduction


Monkeypox was discovered in humans in 1970 in the Democratic Republic of the Congo. Since then, it has become endemic in western and central Africa, and most cases outside that region have been linked to travel or imported animals. In May 2022, cases began appearing in Europe and around the world with no known link to western or central Africa. To date, there have been over 21,000 monkeypox cases in the US and over 55,000 in the world as a result of this ongoing outbreak.


Interest in wastewater-based epidemiology has increased dramatically in recent years as it has been used to track the SARS-CoV-2 virus in communities. There is growing interest in tracking the monkeypox virus through wastewater as well.


Ceres Nanotrap Microbiome A Particles capture and concentrate a wide range of viral and bacterial pathogens present in wastewater. In this study, we demonstrate that Nanotrap Microbiome A Particles bind and concentrate monkeypox virus from wastewater using both manual and automated workflows.







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