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Improved detection of herpesviruses from diluted vitreous specimens using hydrogel particles

POSTER presented at ASM CVS 2022

Infectious uveitis is a serious, sight-threatening intraocular infection that is commonly caused by a variety of herpesviruses. Molecular testing is performed on intraocular fluids for etiology confirmation and proper clinical management. In many cases, vitreous fluid is collected and diluted in large volumes of buffered saline, which impacts sensitivity of pathogen detection. Nanotrap® particles (Ceres Nanosciences) are hydrogel particles functionalized with affinity baits that capture and concentrate analytes, including viruses such as Zika virus, chikungunya, dengue, HIV-1, influenza, respiratory syncytial virus and coronavirus. Here we explore the use of Nanotrap® particles to capture and concentrate low abundant herpesviruses from diluted porcine vitreous specimens.

Nanotrap® particles demonstrated affinity to HSV-1, HSV-2, VZV and CMV in a vitreous matrix (Figure 1). Serially diluted specimens containing virus from 104 to 101 copies/mL were PCR positive after enrichment with Nanotrap® Custom Red Particles. Sensitivity of pathogen detection in the diluted vitreous increased 10-fold when compared with non-enriched specimens.

Nanotrap® particles can capture and concentrate HSV-1, HSV-2, VZV and CMV in a vitreous matrix. Our data demonstrate that these particles can be used for pre-processing of diluted vitreous specimens for improved detection of uveitis-causing herpesviruses.


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