Utilizing Nanotrap® Particles to Improve Nanopore Sequencing of SARS-CoV-2 in VTM

POSTER presented at ASM CVS 2022



The emergence of novel SARS-CoV-2 variants has highlighted the need for accurate, rapid, and deployable sequencing methods. Sample enrichment technologies are particularly relevant, given the requirement for high viral loads to generate deep read depths and significant genome coverage for accurate mutation detection.


Using Nanotrap® Magnetic Virus Particles to capture and concentrate virus prior to RNA extraction improved the sequencing results of SARS-CoV-2 in transport medium samples when compared to RNA extraction methods without enrichment. For contrived samples, using Nanotrap® Magnetic Virus Particles improved viral mapped reads of both extraction methods by 4-fold at 1x106 TCID50/mL and 2-fold at 1x105 TCID50/mL.


>> Significant improvements were observed across 10 SARS-CoV-2 positive diagnostic remnant samples using Ceres Method 1. Using Nanotrap® Magnetic Virus Particles with the QIAmp® Viral RNA Mini Kit, viral mapped reads increased by 7-fold and viral genome coverage increased by 52%, on average.


>> Significant improvements were observed across 10 SARS-CoV-2 positive diagnostic remnant samples using Ceres Method 2 on a KingFisher System. Using Nanotrap® Magnetic Virus Particles with the MagMAXTM Viral/Pathogen Kit increased viral mapped reads by 42-fold and viral genome coverage by 51%, on average.





Related Posts

See All