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App Note: Nanotrap® Enhancement Reagent 1 Improves Detection of Viral RNA from Wastewater Samples

Updated: Oct 26, 2021


Key Advantages

>> Nanotrap® Enhancement Reagent 1 improves SARS-CoV-2 RNA detection in wastewater by at least 1-2 Ct values.

>> Compatible with RNA extraction kits from multiple vendors.

>> Automated methods do not add time to workflow.

>> Outperforms other magnetic virus binding beads and standard concentration methods.


According to the Centers for Disease Control and Prevention, SARS-CoV-2 can be shed in the feces of individuals with asymptomatic or symptomatic infections, making SARS-CoV-2 RNA detection in sewage a COVID-19 indicator that is independent of healthcare-seeking behaviors and access to clinical testing. Due to low viral concentrations and presence of PCR inhibitors in sewage samples, SARS-CoV-2 viral particles must be concentrated prior to nucleic acid isolation. Common approaches for viral particle concentration include HA filtration, skim milk and PEG precipitation methods. These approaches are time consuming and cumbersome.

Nanotrap® Magnetic Virus particles have already been demonstrated to enable sensitive, rapid, and easy-to-use automated and manual methods for wastewater SARS-CoV-2 testing.

A new reagent from Ceres Nanosciences, Nanotrap® Enhancement Reagent 1 (ER1), improves the binding of viruses to Nanotrap® Magnetic Virus Particles in wastewater samples, thus improving downstream detection of viral RNA. This simple and sensitive method is compatible with magnetic bead RNA extraction kits from MACHERY-NAGEL and ThermoFisher.

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